Gene description for TESC
Gene name tescalcin
Gene symbol TESC
Other names/aliases CHP3
TSC
Species Homo sapiens
 Database cross references - TESC
ExoCarta ExoCarta_54997
Entrez Gene 54997
HGNC 26065
MIM 611585
UniProt Q96BS2  
 TESC identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for TESC
Molecular Function
    calcium ion binding GO:0005509 IDA
    protein homodimerization activity GO:0042803 ISS
    protein binding GO:0005515 IPI
    phosphatase inhibitor activity GO:0019212 ISS
    magnesium ion binding GO:0000287 ISS
    protein kinase inhibitor activity GO:0004860 IEA
Biological Process
    negative regulation of protein kinase activity GO:0006469 IEA
    protein maturation GO:0051604 IDA
    positive regulation of granulocyte differentiation GO:0030854 IDA
    male gonad development GO:0008584 IEA
    regulation of cell adhesion mediated by integrin GO:0033628 IMP
    megakaryocyte differentiation GO:0030219 IEA
    positive regulation of gene expression GO:0010628 IMP
    negative regulation of cell proliferation GO:0008285 IMP
    positive regulation of transcription, DNA-templated GO:0045893 IMP
    cellular response to retinoic acid GO:0071300 IDA
    protein stabilization GO:0050821 IDA
    positive regulation of megakaryocyte differentiation GO:0045654 IMP
    protein targeting to plasma membrane GO:0072661 IDA
    positive regulation of sodium:proton antiporter activity GO:0032417 IDA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    plasma membrane GO:0005886 IDA
    lamellipodium GO:0030027 IDA
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 ISS
    ruffle membrane GO:0032587 IEA
    ruffle GO:0001726 ISS
 Experiment description of studies that identified TESC in exosomes
1
Experiment ID 234
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
3
Experiment ID 231
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 232
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 233
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TESC
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAI1 10743
Two-hybrid Homo sapiens
2 TCTE1L  
Two-hybrid Homo sapiens
3 TESC 54997
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 HMG20A  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
5 FLJ11088  
Two-hybrid Homo sapiens
6 SLC9A1 6548
Affinity Capture-MS Homo sapiens
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which TESC is involved
No pathways found





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