Gene description for SATB1
Gene name SATB homeobox 1
Gene symbol SATB1
Other names/aliases -
Species Homo sapiens
 Database cross references - SATB1
ExoCarta ExoCarta_6304
Vesiclepedia VP_6304
Entrez Gene 6304
HGNC 10541
MIM 602075
UniProt Q01826  
 SATB1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Thymus 23844026    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for SATB1
Molecular Function
    chromatin binding GO:0003682 IEA
    protein binding GO:0005515 IPI
    RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription GO:0001227 IDA
    RNA polymerase II regulatory region sequence-specific DNA binding GO:0000977 IDA
    double-stranded DNA binding GO:0003690 TAS
Biological Process
    programmed cell death GO:0012501 TAS
    chromatin remodeling GO:0006338 IEA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IMP
    reflex GO:0060004 IEA
    CD4-positive, alpha-beta T cell differentiation GO:0043367 IEA
    chromatin organization GO:0006325 TAS
    CD8-positive, alpha-beta T cell differentiation GO:0043374 IEA
    transcription, DNA-templated GO:0006351 IEA
    epidermis development GO:0008544 IEA
    viral process GO:0016032 IEA
    apoptotic process GO:0006915 TAS
    cellular component disassembly involved in execution phase of apoptosis GO:0006921 TAS
    histone methylation GO:0016571 IEA
    activated T cell proliferation GO:0050798 IEA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    nucleus GO:0005634 IDA
    nuclear heterochromatin GO:0005720 IEA
    nuclear matrix GO:0016363 IEA
    PML body GO:0016605 IDA
 Experiment description of studies that identified SATB1 in exosomes
1
Experiment ID 1203
MISEV standards
✔
EM
EV Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 193
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|CD9
EV Enriched markers
✔
PHB
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 194
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 195
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SATB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BAZ1A 11177
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
2 CUX1 1523
Invivo Homo sapiens
Invitro Homo sapiens
3 UBE2I 7329
Two-hybrid Homo sapiens
4 CHD4 1108
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
5 HDAC2 3066
Co-fractionation Homo sapiens
6 MTA2 9219
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
7 HDAC1 3065
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
8 POLR2J  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
9 SIN3A  
Co-fractionation Homo sapiens
10 SATB1 6304
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 SMARCA5 8467
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which SATB1 is involved
PathwayEvidenceSource
Apoptotic cleavage of cellular proteins TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here