Gene description for Pls3
Gene name plastin 3 (T-isoform)
Gene symbol Pls3
Other names/aliases AI115446
AL024105
T-fimbrin
Species Mus musculus
 Database cross references - Pls3
ExoCarta ExoCarta_102866
Vesiclepedia VP_102866
Entrez Gene 102866
UniProt Q99K51  
 Pls3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pls3
Molecular Function
    calcium ion binding GO:0005509 IEA
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 IEA
    structural constituent of presynaptic actin cytoskeleton GO:0098699 IDA
    structural constituent of presynaptic actin cytoskeleton GO:0098699 IMP
Biological Process
    actin filament bundle assembly GO:0051017 IBA
    actin filament bundle assembly GO:0051017 IEA
    actin filament network formation GO:0051639 IBA
    bone development GO:0060348 ISO
    regulation of synaptic vesicle cycle GO:0098693 IDA
    regulation of synaptic vesicle cycle GO:0098693 IMP
    presynaptic actin cytoskeleton organization GO:0099140 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 ISO
    actin filament GO:0005884 IBA
    plasma membrane GO:0005886 ISO
    neuromuscular junction GO:0031594 IDA
    neuromuscular junction GO:0031594 IMP
    stereocilium GO:0032420 ISO
    actin filament bundle GO:0032432 IBA
 Experiment description of studies that identified Pls3 in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pls3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Stau1  
Co-fractionation Mus musculus
3 Kcna3  
Affinity Capture-MS Mus musculus
4 Eed  
Affinity Capture-MS Mus musculus
5 Usp7  
Affinity Capture-MS Mus musculus
6 Atxn2 20239
Affinity Capture-Western Mus musculus
7 Slc25a4 11739
Cross-Linking-MS (XL-MS) Mus musculus
8 Unk  
Affinity Capture-RNA Mus musculus
View the network image/svg+xml
 Pathways in which Pls3 is involved
No pathways found





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