Gene description for Ndufa8
Gene name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8
Gene symbol Ndufa8
Other names/aliases 0610033L03Rik
AW261656
Species Mus musculus
 Database cross references - Ndufa8
ExoCarta ExoCarta_68375
Vesiclepedia VP_68375
Entrez Gene 68375
UniProt Q9DCJ5  
 Ndufa8 identified in sEVs derived from the following tissue/cell type
Melanoma cells 34957415    
Melanoma cells 34957415    
 Gene ontology annotations for Ndufa8
Molecular Function
    protein-containing complex binding GO:0044877 ISO
Biological Process
    mitochondrial electron transport, NADH to ubiquinone GO:0006120 IEA
    aerobic respiration GO:0009060 NAS
    mitochondrial respiratory chain complex I assembly GO:0032981 ISO
    mitochondrial respiratory chain complex I assembly GO:0032981 ISS
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 NAS
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 ISO
    mitochondrial inner membrane GO:0005743 HDA
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 ISO
    mitochondrial intermembrane space GO:0005758 IEA
    mitochondrial intermembrane space GO:0005758 ISO
    respiratory chain complex I GO:0045271 IBA
    respiratory chain complex I GO:0045271 IDA
    respiratory chain complex I GO:0045271 ISO
    respiratory chain complex I GO:0045271 ISS
 Experiment description of studies that identified Ndufa8 in sEVs
1
Experiment ID 1117
MISEV standards
Biophysical techniques
Cd81|Icam1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 1118
MISEV standards
Biophysical techniques
Cd81|Icam1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ndufa8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ndufa12  
Co-fractionation Mus musculus
2 Ndufa8 68375
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Aifm1  
Cross-Linking-MS (XL-MS) Mus musculus
5 Chchd3  
Co-fractionation Mus musculus
6 SNCA 6622
Affinity Capture-MS Homo sapiens
7 Atp5j  
Co-fractionation Mus musculus
8 Lmo2  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
9 Eed  
Affinity Capture-MS Mus musculus
10 Ndufb5  
Cross-Linking-MS (XL-MS) Mus musculus
11 Coq9  
Co-fractionation Mus musculus
12 Atp5d  
Co-fractionation Mus musculus
13 Ndufc2  
Cross-Linking-MS (XL-MS) Mus musculus
14 Htt  
Affinity Capture-MS Mus musculus
15 Cyct  
Cross-Linking-MS (XL-MS) Mus musculus
16 Slc25a4 11739
Cross-Linking-MS (XL-MS) Mus musculus
17 Ckmt2  
Cross-Linking-MS (XL-MS) Mus musculus
18 Sucla2  
Co-fractionation Mus musculus
19 Fancd2  
Affinity Capture-MS Mus musculus
20 Cycs 13063
Cross-Linking-MS (XL-MS) Mus musculus
21 Suclg1  
Co-fractionation Mus musculus
View the network image/svg+xml



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