Gene description for Cycs
Gene name cytochrome c, somatic
Gene symbol Cycs
Other names/aliases -
Species Mus musculus
 Database cross references - Cycs
ExoCarta ExoCarta_13063
Vesiclepedia VP_13063
Entrez Gene 13063
UniProt P62897  
 Cycs identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Cycs
Molecular Function
    electron transfer activity GO:0009055 IBA
    electron transfer activity GO:0009055 IEA
    enzyme binding GO:0019899 ISO
    heme binding GO:0020037 IDA
    metal ion binding GO:0046872 IEA
Biological Process
    mitochondrial electron transport, ubiquinol to cytochrome c GO:0006122 IBA
    mitochondrial electron transport, cytochrome c to oxygen GO:0006123 IBA
    apoptotic process GO:0006915 IDA
    hydrogen peroxide metabolic process GO:0042743 IDA
    regulation of protein binding GO:0043393 IMP
    intrinsic apoptotic signaling pathway GO:0097193 NAS
    positive regulation of cysteine-type endopeptidase activity GO:2001056 IMP
Subcellular Localization
    nucleus GO:0005634 ISO
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISO
    mitochondrial intermembrane space GO:0005758 IBA
    mitochondrial intermembrane space GO:0005758 IEA
    mitochondrial intermembrane space GO:0005758 ISO
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    myelin sheath GO:0043209 HDA
    apoptosome GO:0043293 ISO
 Experiment description of studies that identified Cycs in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Cycs
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rnf7  
Co-fractionation Mus musculus
2 Cox4i1 12857
Cross-Linking-MS (XL-MS) Mus musculus
3 Cox6b1  
Cross-Linking-MS (XL-MS) Mus musculus
4 Apoo  
Cross-Linking-MS (XL-MS) Mus musculus
5 Ndufb5  
Co-fractionation Mus musculus
6 Slc25a13 50799
Cross-Linking-MS (XL-MS) Mus musculus
7 Slc25a4 11739
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
8 Ndufv2  
Co-fractionation Mus musculus
9 Ckmt2  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
10 Cycs 13063
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
11 Uqcrh  
Cross-Linking-MS (XL-MS) Mus musculus
Co-fractionation Mus musculus
12 Aifm1  
Cross-Linking-MS (XL-MS) Mus musculus
13 Uqcrc1  
Co-fractionation Mus musculus
14 Usmg5  
Co-fractionation Mus musculus
15 Kctd13  
Affinity Capture-MS Mus musculus
16 Cox6c  
Cross-Linking-MS (XL-MS) Mus musculus
17 Cox7b  
Cross-Linking-MS (XL-MS) Mus musculus
18 Vdac1 22333
Co-fractionation Mus musculus
19 Atp5k  
Cross-Linking-MS (XL-MS) Mus musculus
20 Ndufa8 68375
Cross-Linking-MS (XL-MS) Mus musculus
21 Slc25a12  
Cross-Linking-MS (XL-MS) Mus musculus
22 Immt  
Cross-Linking-MS (XL-MS) Mus musculus
23 Pfkm 18642
Co-fractionation Mus musculus
24 Sdha 66945
Co-fractionation Mus musculus
25 Cyct  
Cross-Linking-MS (XL-MS) Mus musculus
26 Suclg1  
Co-fractionation Mus musculus
27 Ndufa12  
Co-fractionation Mus musculus
28 Atp5f1  
Co-fractionation Mus musculus
29 Chchd3  
Cross-Linking-MS (XL-MS) Mus musculus
30 Uqcrq  
Cross-Linking-MS (XL-MS) Mus musculus
31 Sdhb 67680
Co-fractionation Mus musculus
32 Uqcr10  
Co-fractionation Mus musculus
33 Uqcrc2 67003
Co-fractionation Mus musculus
34 Ndufb6  
Co-fractionation Mus musculus
35 Uqcrfs1 66694
Cross-Linking-MS (XL-MS) Mus musculus
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
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