Gene description for Vdac1
Gene name voltage-dependent anion channel 1
Gene symbol Vdac1
Other names/aliases AL033343
Vdac5
Species Mus musculus
 Database cross references - Vdac1
ExoCarta ExoCarta_22333
Vesiclepedia VP_22333
Entrez Gene 22333
UniProt Q60932  
 Vdac1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Vdac1
Molecular Function
    voltage-gated monoatomic ion channel activity GO:0005244 ISS
    monoatomic anion channel activity GO:0005253 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    oxysterol binding GO:0008142 IMP
    voltage-gated monoatomic anion channel activity GO:0008308 IBA
    voltage-gated monoatomic anion channel activity GO:0008308 IDA
    voltage-gated monoatomic anion channel activity GO:0008308 ISO
    porin activity GO:0015288 IEA
    cholesterol binding GO:0015485 IEA
    cholesterol binding GO:0015485 ISO
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    phosphatidylcholine binding GO:0031210 IEA
    phosphatidylcholine binding GO:0031210 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    transmembrane transporter binding GO:0044325 IEA
    transmembrane transporter binding GO:0044325 ISO
    protein-containing complex binding GO:0044877 ISO
    ceramide binding GO:0097001 IEA
    ceramide binding GO:0097001 ISO
Biological Process
    behavioral fear response GO:0001662 IMP
    acetyl-CoA biosynthetic process from pyruvate GO:0006086 ISO
    monoatomic anion transport GO:0006820 ISO
    mitochondrial calcium ion transmembrane transport GO:0006851 ISO
    lipid transport GO:0006869 IEA
    apoptotic process GO:0006915 ISO
    apoptotic process GO:0006915 ISS
    chemical synaptic transmission GO:0007268 IMP
    neuron-neuron synaptic transmission GO:0007270 IMP
    learning GO:0007612 IMP
    epithelial cell differentiation GO:0030855 IEA
    calcium import into the mitochondrion GO:0036444 ISS
    positive regulation of apoptotic process GO:0043065 IEA
    negative regulation of apoptotic process GO:0043066 IEA
    negative regulation of apoptotic process GO:0043066 ISO
    monoatomic anion transmembrane transport GO:0098656 IEA
    negative regulation of calcium import into the mitochondrion GO:0110099 IEA
    negative regulation of calcium import into the mitochondrion GO:0110099 ISO
    regulation of mitophagy GO:1901524 IMP
    positive regulation of type 2 mitophagy GO:1905091 IEA
    positive regulation of type 2 mitophagy GO:1905091 ISO
    negative regulation of reactive oxygen species metabolic process GO:2000378 IMP
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISO
    mitochondrial outer membrane GO:0005741 IBA
    mitochondrial outer membrane GO:0005741 ISO
    mitochondrial outer membrane GO:0005741 ISS
    mitochondrial inner membrane GO:0005743 HDA
    mitochondrial inner membrane GO:0005743 ISS
    mitochondrial permeability transition pore complex GO:0005757 ISO
    mitochondrial permeability transition pore complex GO:0005757 ISS
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    synaptic vesicle GO:0008021 ISO
    membrane GO:0016020 IDA
    membrane GO:0016020 ISO
    mitochondrial membrane GO:0031966 ISO
    protein-containing complex GO:0032991 ISO
    mitochondrial nucleoid GO:0042645 IEA
    mitochondrial nucleoid GO:0042645 ISO
    myelin sheath GO:0043209 HDA
    membrane raft GO:0045121 IEA
    synapse GO:0045202 IEA
    presynaptic active zone membrane GO:0048787 ISO
    postsynaptic density membrane GO:0098839 ISO
 Experiment description of studies that identified Vdac1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Vdac1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cpt1b  
Cross-Linking-MS (XL-MS) Mus musculus
2 Prkce  
Co-fractionation Mus musculus
3 Tomm20 67952
Co-fractionation Mus musculus
4 Tuba4a 22145
Co-fractionation Mus musculus
5 SNCA 6622
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
6 Kcna3  
Affinity Capture-MS Mus musculus
7 Eed  
Affinity Capture-MS Mus musculus
8 Dynlt3 67117
Co-fractionation Mus musculus
9 Csnk2b 13001
Co-fractionation Mus musculus
10 Mapt  
Affinity Capture-MS Mus musculus
11 Vdac1 22333
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
12 Htt  
Affinity Capture-MS Mus musculus
13 Ckmt1  
Co-fractionation Mus musculus
14 Tspo  
Affinity Capture-Western Mus musculus
15 Nedd4 17999
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
16 Tia1  
Affinity Capture-MS Mus musculus
17 Kctd13  
Affinity Capture-MS Mus musculus
18 Cycs 13063
Co-fractionation Mus musculus
19 Kcnma1  
Affinity Capture-MS Mus musculus
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