Gene description for Tomm20
Gene name translocase of outer mitochondrial membrane 20 homolog (yeast)
Gene symbol Tomm20
Other names/aliases 1810060K07Rik
BB284719
Gm19268
MAS20
MOM19
TOM20
mKIAA0016
Species Mus musculus
 Database cross references - Tomm20
ExoCarta ExoCarta_67952
Vesiclepedia VP_67952
Entrez Gene 67952
UniProt Q9DCC8  
 Tomm20 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
Melanoma cells 34957415    
Melanoma cells 34957415    
Neural stem cells 25242146    
Osteosarcoma cells 33785738    
Osteosarcoma cells 33785738    
 Gene ontology annotations for Tomm20
Molecular Function
    protein binding GO:0005515 IPI
    protein transmembrane transporter activity GO:0008320 IBA
    protein-transporting ATPase activity GO:0015450 ISO
    protein-transporting ATPase activity GO:0015450 ISS
    mitochondrion targeting sequence binding GO:0030943 IBA
    mitochondrion targeting sequence binding GO:0030943 IEA
    mitochondrion targeting sequence binding GO:0030943 ISO
    unfolded protein binding GO:0051082 ISO
    unfolded protein binding GO:0051082 ISS
Biological Process
    protein targeting to mitochondrion GO:0006626 ISO
    protein targeting to mitochondrion GO:0006626 ISS
    intracellular protein transport GO:0006886 IEA
    response to muscle activity GO:0014850 IEA
    tRNA import into mitochondrion GO:0016031 IBA
    protein import into mitochondrial matrix GO:0030150 IBA
    protein transmembrane transport GO:0071806 IEA
    response to 3,3',5-triiodo-L-thyronine GO:1905242 IEA
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 ISO
    mitochondrial envelope GO:0005740 IDA
    mitochondrial outer membrane GO:0005741 ISO
    mitochondrial outer membrane translocase complex GO:0005742 IBA
    mitochondrial outer membrane translocase complex GO:0005742 IEA
    mitochondria-associated endoplasmic reticulum membrane contact site GO:0044233 ISO
    cell periphery GO:0071944 IDA
    sperm midpiece GO:0097225 IDA
    migrasome GO:0140494 IDA
 Experiment description of studies that identified Tomm20 in sEVs
1
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 1117
MISEV standards
✘ Biophysical techniques
✔
Cd81|Icam1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 1118
MISEV standards
✘ Biophysical techniques
✔
Cd81|Icam1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 264
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th2 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 1321
MISEV standards
Biophysical techniques
✘ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33785738    
Organism Homo sapiens
Experiment description Selective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs
Authors "Todkar K, Chikhi L, Desjardins V, El-Mortada F, Pépin G, Germain M. "
Journal name Nat Commun
Publication year 2021
Sample Osteosarcoma cells
Sample name U2OS
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
6
Experiment ID 1322
MISEV standards
Biophysical techniques
✘ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33785738    
Organism Homo sapiens
Experiment description Selective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs
Authors "Todkar K, Chikhi L, Desjardins V, El-Mortada F, Pépin G, Germain M. "
Journal name Nat Commun
Publication year 2021
Sample Osteosarcoma cells
Sample name U2OS
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
 Protein-protein interactions for Tomm20
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Vdac1 22333
Co-fractionation Mus musculus
2 Eed  
Affinity Capture-MS Mus musculus
3 Atg16l1  
Affinity Capture-MS Mus musculus
4 Tmem173  
Proximity Label-MS Mus musculus
View the network image/svg+xml



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