Gene description for Tomm20
Gene name translocase of outer mitochondrial membrane 20 homolog (yeast)
Gene symbol Tomm20
Other names/aliases 1810060K07Rik
BB284719
Gm19268
MAS20
MOM19
TOM20
mKIAA0016
Species Mus musculus
 Database cross references - Tomm20
ExoCarta ExoCarta_67952
Entrez Gene 67952
UniProt Q9DCC8  
 Tomm20 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
Neural stem cells 25242146    
 Gene ontology annotations for Tomm20
Molecular Function
    P-P-bond-hydrolysis-driven protein transmembrane transporter activity GO:0015450 ISO
    unfolded protein binding GO:0051082 ISO
Biological Process
    protein transmembrane transport GO:0071806 ISO
    protein targeting to mitochondrion GO:0006626 ISO
    transport GO:0006810 IEA
    protein transport GO:0015031 IEA
    intracellular protein transport GO:0006886 IEA
    protein targeting GO:0006605 IEA
Subcellular Localization
    ER-mitochondrion membrane contact site GO:0044233 ISO
    integral component of membrane GO:0016021 IEA
    mitochondrion GO:0005739 ISO
    membrane GO:0016020 IEA
    mitochondrial outer membrane GO:0005741 ISO
    mitochondrial outer membrane translocase complex GO:0005742 IEA
    mitochondrial envelope GO:0005740 IDA
 Experiment description of studies that identified Tomm20 in exosomes
1
Experiment ID 15
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 264
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th2 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Tomm20
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Tomm20 is involved
PathwayEvidenceSource
Mitochondrial protein import IEA Reactome





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