Gene description for Csnk2b
Gene name casein kinase 2, beta polypeptide
Gene symbol Csnk2b
Other names/aliases CK II beta
Species Mus musculus
 Database cross references - Csnk2b
ExoCarta ExoCarta_13001
Vesiclepedia VP_13001
Entrez Gene 13001
UniProt P67871  
 Csnk2b identified in sEVs derived from the following tissue/cell type
Basophilic leukemia cells 20424270    
Colon cancer cells 37309723    
Mast cells 17486113    
Neural stem cells 25242146    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Csnk2b
Molecular Function
    chromatin binding GO:0003682 ISO
    protein serine/threonine kinase activity GO:0004674 ISO
    signaling receptor binding GO:0005102 ISO
    protein binding GO:0005515 IPI
    protein kinase regulator activity GO:0019887 IBA
    protein kinase regulator activity GO:0019887 IEA
    protein domain specific binding GO:0019904 ISO
    protein-macromolecule adaptor activity GO:0030674 ISO
    identical protein binding GO:0042802 ISO
    ribonucleoprotein complex binding GO:0043021 ISO
    metal ion binding GO:0046872 IEA
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 ISO
Biological Process
    Wnt signaling pathway GO:0016055 IEA
    peptidyl-threonine phosphorylation GO:0018107 ISO
    negative regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032435 ISO
    positive regulation of activin receptor signaling pathway GO:0032927 ISO
    adiponectin-activated signaling pathway GO:0033211 ISO
    negative regulation of blood vessel endothelial cell migration GO:0043537 ISO
    positive regulation of SMAD protein signal transduction GO:0060391 ISO
    endothelial tube morphogenesis GO:0061154 ISO
    symbiont-mediated disruption of host cell PML body GO:0075342 ISO
    negative regulation of viral life cycle GO:1903901 ISO
Subcellular Localization
    chromatin GO:0000785 ISO
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 ISO
    protein kinase CK2 complex GO:0005956 IBA
    protein kinase CK2 complex GO:0005956 IEA
    protein kinase CK2 complex GO:0005956 ISO
    nuclear matrix GO:0016363 ISO
    PML body GO:0016605 ISO
    PcG protein complex GO:0031519 ISO
    PcG protein complex GO:0031519 ISS
    cell projection GO:0042995 ISO
 Experiment description of studies that identified Csnk2b in sEVs
1
Experiment ID 89
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20424270    
Organism Rattus norvegicus
Experiment description Exosomes account for vesicle-mediated transcellular transport of activatable phospholipases and prostaglandins.
Authors "Subra C, Grand D, Laulagnier K, Stella A, Lambeau G, Paillasse M, De Medina P, Monsarrat B, Perret B, Silvente-Poirot S, Poirot M, Record M."
Journal name JLR
Publication year 2010
Sample Basophilic leukemia cells
Sample name RBL-2H3
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Gas chromatography
Thin layer chromatography
Mass spectrometry [Qstar XL]
FACS
2
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 263
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Csnk2b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rnf2  
Affinity Capture-MS Mus musculus
2 Atg16l1  
Affinity Capture-MS Mus musculus
3 Uso1 56041
Co-fractionation Mus musculus
4 Upf1 19704
Co-fractionation Mus musculus
5 Vdac1 22333
Co-fractionation Mus musculus
6 Tcte3  
Two-hybrid Mus musculus
7 Shank3  
Affinity Capture-MS Mus musculus
8 CSNK2A1 1457
Two-hybrid Homo sapiens
9 Unk  
Affinity Capture-RNA Mus musculus
10 Mecp2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Csnk2b is involved
PathwayEvidenceSource
Autophagy IEA Reactome
Axon guidance IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Chaperonin-mediated protein folding IEA Reactome
Condensation of Prometaphase Chromosomes IEA Reactome
Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding IEA Reactome
Developmental Biology IEA Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway IEA Reactome
Glycerophospholipid biosynthesis IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
Intracellular signaling by second messengers IEA Reactome
L1CAM interactions IEA Reactome
M Phase IEA Reactome
Macroautophagy IEA Reactome
Metabolism IEA Reactome
Metabolism of lipids IEA Reactome
Metabolism of proteins IEA Reactome
Mitophagy IEA Reactome
Mitotic Prometaphase IEA Reactome
Nervous system development IEA Reactome
Neutrophil degranulation IEA Reactome
Phospholipid metabolism IEA Reactome
PIP3 activates AKT signaling IEA Reactome
Protein folding IEA Reactome
PTEN Regulation IEA Reactome
Receptor Mediated Mitophagy IEA Reactome
Regulation of PTEN stability and activity IEA Reactome
Regulation of TP53 Activity IEA Reactome
Regulation of TP53 Activity through Phosphorylation IEA Reactome
RNA Polymerase II Transcription IEA Reactome
RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known IEA Reactome
Selective autophagy IEA Reactome
Signal Transduction IEA Reactome
Signal transduction by L1 IEA Reactome
Signaling by WNT IEA Reactome
Synthesis of PC IEA Reactome
TCF dependent signaling in response to WNT IEA Reactome
Transcriptional regulation by RUNX1 IEA Reactome
Transcriptional Regulation by TP53 IEA Reactome
WNT mediated activation of DVL IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here