Gene description for Nedd4
Gene name neural precursor cell expressed, developmentally down-regulated 4
Gene symbol Nedd4
Other names/aliases AA959633
AL023035
AU019897
E430025J12Rik
Nedd4-1
Nedd4a
mKIAA0093
Species Mus musculus
 Database cross references - Nedd4
ExoCarta ExoCarta_17999
Vesiclepedia VP_17999
Entrez Gene 17999
UniProt P46935  
 Nedd4 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Nedd4
Molecular Function
    protein-glycine ligase activity, elongating GO:0070737 IEA
    protein-glycine ligase activity GO:0070735 IEA
    protein binding GO:0005515 IPI
    ligase activity GO:0016874 IEA
    protein-glutamic acid ligase activity GO:0070739 IEA
    coenzyme F420-0 gamma-glutamyl ligase activity GO:0043773 IEA
    phosphoserine binding GO:0050815 IDA
    sodium channel inhibitor activity GO:0019871 IDA
    protein C-terminus binding GO:0008022 ISO
    UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity GO:0008766 IEA
    ubiquitin-protein transferase activity GO:0004842 ISO
    tubulin-glycine ligase activity GO:0070738 IEA
    tubulin-glutamic acid ligase activity GO:0070740 IEA
    phosphothreonine binding GO:0050816 IDA
    ribosomal S6-glutamic acid ligase activity GO:0018169 IEA
    ubiquitin protein ligase activity GO:0061630 IDA
    protein-glycine ligase activity, initiating GO:0070736 IEA
    coenzyme F420-2 alpha-glutamyl ligase activity GO:0043774 IEA
    proline-rich region binding GO:0070064 IPI
Biological Process
    nervous system development GO:0007399 IEA
    negative regulation of sodium ion transport GO:0010766 IDA
    adaptive immune response GO:0002250 IMP
    outflow tract morphogenesis GO:0003151 IMP
    protein monoubiquitination GO:0006513 IDA
    blood vessel morphogenesis GO:0048514 IMP
    protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:0042787 IMP
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IMP
    neuromuscular junction development GO:0007528 IMP
    regulation of synapse organization GO:0050807 IMP
    negative regulation of vascular endothelial growth factor receptor signaling pathway GO:0030948 IDA
    T cell activation GO:0042110 IMP
    endocardial cushion development GO:0003197 IMP
    positive regulation of protein catabolic process GO:0045732 ISO
    protein K63-linked ubiquitination GO:0070534 ISO
    regulation of dendrite morphogenesis GO:0048814 ISO
    protein ubiquitination GO:0016567 IDA
Subcellular Localization
    membrane raft GO:0045121 ISO
    microvillus GO:0005902 ISO
    cytosol GO:0005829 IDA
    ubiquitin ligase complex GO:0000151 IPI
    membrane GO:0016020 IDA
    plasma membrane GO:0005886 IEA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified Nedd4 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Nedd4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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