Gene description for Hk1
Gene name hexokinase 1
Gene symbol Hk1
Other names/aliases BB404130
Hk-1
Hk1-s
dea
mHk1-s
Species Mus musculus
 Database cross references - Hk1
ExoCarta ExoCarta_15275
Vesiclepedia VP_15275
Entrez Gene 15275
UniProt P17710  
 Hk1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hk1
Molecular Function
    nucleotide binding GO:0000166 IEA
    fructokinase activity GO:0008865 IBA
    protein kinase activity GO:0004672 ISO
    hexokinase activity GO:0004396 TAS
    ATP binding GO:0005524 ISO
    glucose binding GO:0005536 ISO
    mannokinase activity GO:0019158 IBA
    protein binding GO:0005515 IPI
    protein homodimerization activity GO:0042803 ISO
    protein complex binding GO:0032403 ISO
    glucokinase activity GO:0004340 ISO
    phosphotransferase activity, alcohol group as acceptor GO:0016773 IEA
    kinase activity GO:0016301 IEA
    catalytic activity GO:0003824 IEA
    transferase activity GO:0016740 IEA
Biological Process
    phosphorylation GO:0016310 IEA
    canonical glycolysis GO:0061621 IC
    response to ischemia GO:0002931 ISO
    glycolytic process GO:0006096 TAS
    metabolic process GO:0008152 IEA
    negative regulation of apoptotic process GO:0043066 ISO
    cellular glucose homeostasis GO:0001678 IBA
    carbohydrate metabolic process GO:0005975 IEA
    regulation of anion channel activity GO:0010359 ISO
    peptidyl-threonine phosphorylation GO:0018107 ISO
    protein phosphorylation GO:0006468 ISO
    protein autophosphorylation GO:0046777 ISO
    peptidyl-tyrosine phosphorylation GO:0018108 ISO
    glucose 6-phosphate metabolic process GO:0051156 ISO
    carbohydrate phosphorylation GO:0046835 ISO
    peptidyl-serine phosphorylation GO:0018105 ISO
Subcellular Localization
    protein complex GO:0043234 ISO
    sperm principal piece GO:0097228 IDA
    caveola GO:0005901 ISO
    membrane raft GO:0045121 IDA
    mitochondrial outer membrane GO:0005741 IEA
    cilium GO:0005929 IDA
    cell GO:0005623 IBA
    mitochondrion GO:0005739 ISO
    cytosol GO:0005829 ISO
    membrane GO:0016020 IEA
 Experiment description of studies that identified Hk1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hk1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Hk1 is involved
PathwayEvidenceSource
Glucose transport IEA Reactome
Glycolysis IEA Reactome





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