Gene description for Gsn
Gene name gelsolin
Gene symbol Gsn
Other names/aliases ADF
Species Mus musculus
 Database cross references - Gsn
ExoCarta ExoCarta_227753
Vesiclepedia VP_227753
Entrez Gene 227753
UniProt P13020  
 Gsn identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
Mast cells 17486113    
Oligodendrocytes 21136642    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Gsn
Molecular Function
    actin binding GO:0003779 ISO
    protein domain specific binding GO:0019904 ISO
    metal ion binding GO:0046872 IEA
    myosin II binding GO:0045159 ISO
    protein binding GO:0005515 IPI
    calcium ion binding GO:0005509 ISO
Biological Process
    barbed-end actin filament capping GO:0051016 IEA
    regulation of wound healing, spreading of epidermal cells GO:1903689 ISO
    positive regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway GO:2001269 ISO
    negative regulation of viral entry into host cell GO:0046597 ISO
    regulation of receptor clustering GO:1903909 ISO
    positive regulation of keratinocyte apoptotic process GO:1902174 ISO
    actin nucleation GO:0045010 IEA
    renal protein absorption GO:0097017 ISO
    cellular response to cadmium ion GO:0071276 ISO
    positive regulation of gene expression GO:0010628 ISO
    regulation of establishment of T cell polarity GO:1903903 ISO
    hepatocyte apoptotic process GO:0097284 ISO
    actin filament reorganization GO:0090527 ISO
    regulation of podosome assembly GO:0071801 ISO
    actin filament polymerization GO:0030041 ISO
    sequestering of actin monomers GO:0042989 ISO
    vesicle-mediated transport GO:0016192 IMP
    protein destabilization GO:0031648 ISO
    amyloid fibril formation GO:1990000 ISO
    regulation of plasma membrane raft polarization GO:1903906 ISO
    apoptotic process GO:0006915 ISO
    actin polymerization or depolymerization GO:0008154 ISO
    cilium morphogenesis GO:0060271 ISO
    actin filament capping GO:0051693 ISO
    regulation of cell adhesion GO:0030155 ISO
    actin filament severing GO:0051014 ISO
    positive regulation of protein processing in phagocytic vesicle GO:1903923 IMP
    positive regulation of actin nucleation GO:0051127 ISO
    cell projection organization GO:0030030 IEA
    phosphatidylinositol-mediated signaling GO:0048015 ISO
    striated muscle atrophy GO:0014891 ISO
    phagocytosis, engulfment GO:0006911 IMP
Subcellular Localization
    myelin sheath GO:0043209 ISO
    actin cytoskeleton GO:0015629 ISO
    extracellular exosome GO:0070062 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    extracellular space GO:0005615 ISO
    sarcoplasm GO:0016528 ISO
    cytoplasm GO:0005737 ISO
    ruffle GO:0001726 ISO
    protein complex GO:0043234 ISO
    cytosol GO:0005829 ISO
    nucleus GO:0005634 ISO
    podosome GO:0002102 ISO
    actin cap GO:0030478 ISO
    cortical actin cytoskeleton GO:0030864 ISO
    plasma membrane GO:0005886 ISO
    focal adhesion GO:0005925 ISO
    extracellular region GO:0005576 ISO
    lamellipodium GO:0030027 IDA
    blood microparticle GO:0072562 ISO
    cytoskeleton GO:0005856 IEA
 Experiment description of studies that identified Gsn in exosomes
1
Experiment ID 907
MISEV standards
EM
EV Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
HSPA5
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
EM
EV Biophysical techniques
HSP70|HSP90
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 15
MISEV standards
EM
EV Biophysical techniques
CD63
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 33
MISEV standards
EM|IEM
EV Biophysical techniques
Alix|TSG101
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
5
Experiment ID 908
MISEV standards
EM
EV Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
HSPA5
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Gsn
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Gsn is involved
PathwayEvidenceSource
Caspase-mediated cleavage of cytoskeletal proteins IEA Reactome





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