Gene description for Kcnab2
Gene name potassium voltage-gated channel, shaker-related subfamily, beta member 2
Gene symbol Kcnab2
Other names/aliases F5
I2rf5
Kcnb3
kv-beta-2
Species Mus musculus
 Database cross references - Kcnab2
ExoCarta ExoCarta_16498
Vesiclepedia VP_16498
Entrez Gene 16498
UniProt P62482  
 Kcnab2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Melanoma cells 34957415    
Melanoma cells 34957415    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Kcnab2
Molecular Function
    aldo-keto reductase (NADPH) activity GO:0004033 IBA
    aldo-keto reductase (NADPH) activity GO:0004033 ISO
    aldo-keto reductase (NADPH) activity GO:0004033 ISS
    voltage-gated potassium channel activity GO:0005249 IEA
    protein binding GO:0005515 IPI
    potassium channel regulator activity GO:0015459 IBA
    potassium channel regulator activity GO:0015459 ISO
    potassium channel regulator activity GO:0015459 ISS
    transmembrane transporter binding GO:0044325 IBA
    transmembrane transporter binding GO:0044325 ISO
    protein-containing complex binding GO:0044877 ISO
    methylglyoxal reductase (NADPH) (acetol producing) activity GO:1990002 IEA
Biological Process
    hematopoietic progenitor cell differentiation GO:0002244 IGI
    neuromuscular process GO:0050905 IMP
    NADPH oxidation GO:0070995 ISO
    NADPH oxidation GO:0070995 ISS
    potassium ion transmembrane transport GO:0071805 IEA
    regulation of potassium ion transmembrane transport GO:1901379 IBA
    regulation of potassium ion transmembrane transport GO:1901379 ISO
    regulation of potassium ion transmembrane transport GO:1901379 ISS
    regulation of protein localization to cell surface GO:2000008 ISO
    regulation of protein localization to cell surface GO:2000008 ISS
Subcellular Localization
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    microtubule GO:0005874 ISO
    microtubule GO:0005874 ISS
    voltage-gated potassium channel complex GO:0008076 IBA
    voltage-gated potassium channel complex GO:0008076 ISO
    cytoplasmic side of plasma membrane GO:0009898 ISO
    cytoplasmic side of plasma membrane GO:0009898 ISS
    postsynaptic density GO:0014069 IDA
    membrane GO:0016020 ISO
    membrane GO:0016020 ISS
    axon GO:0030424 IDA
    axon GO:0030424 ISO
    potassium channel complex GO:0034705 ISO
    potassium channel complex GO:0034705 ISS
    neuron projection GO:0043005 ISO
    axon initial segment GO:0043194 IDA
    axon terminus GO:0043679 IDA
    juxtaparanode region of axon GO:0044224 IBA
    juxtaparanode region of axon GO:0044224 IDA
    juxtaparanode region of axon GO:0044224 ISO
    postsynaptic density membrane GO:0098839 ISO
    glutamatergic synapse GO:0098978 ISO
    pinceau fiber GO:1990031 ISO
    pinceau fiber GO:1990031 ISS
 Experiment description of studies that identified Kcnab2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 1117
MISEV standards
✘ Biophysical techniques
✔
Cd81|Icam1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 1118
MISEV standards
✘ Biophysical techniques
✔
Cd81|Icam1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Kcnab2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kcna2  
FRET Mus musculus
Affinity Capture-Western Mus musculus
2 Syngap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
3 SNCA 6622
Affinity Capture-MS Homo sapiens
4 Kctd13  
Affinity Capture-MS Mus musculus
5 Dlg4  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
6 Ywhaz 22631
Affinity Capture-MS Mus musculus
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