Gene description for Pfkl
Gene name phosphofructokinase, liver, B-type
Gene symbol Pfkl
Other names/aliases AA407869
ATP-PFK
PFK-L
Species Mus musculus
 Database cross references - Pfkl
ExoCarta ExoCarta_18641
Entrez Gene 18641
UniProt P12382  
 Pfkl identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Pfkl
Molecular Function
    monosaccharide binding GO:0048029 ISO
    kinase activity GO:0016301 IEA
    ATP binding GO:0005524 ISO
    fructose binding GO:0070061 ISO
    nucleotide binding GO:0000166 IEA
    identical protein binding GO:0042802 ISO
    fructose-6-phosphate binding GO:0070095 ISO
    metal ion binding GO:0046872 IEA
    transferase activity GO:0016740 IEA
    catalytic activity GO:0003824 IEA
    kinase binding GO:0019900 ISO
    6-phosphofructokinase activity GO:0003872 ISO
Biological Process
    protein oligomerization GO:0051259 ISO
    fructose 6-phosphate metabolic process GO:0006002 ISO
    fructose 1,6-bisphosphate metabolic process GO:0030388 ISO
    phosphorylation GO:0016310 IEA
    glycolytic process GO:0006096 ISO
    metabolic process GO:0008152 IEA
    protein homotetramerization GO:0051289 ISO
    carbohydrate metabolic process GO:0005975 ISO
    response to glucose GO:0009749 ISO
    carbohydrate phosphorylation GO:0046835 ISO
    negative regulation of insulin secretion GO:0046676 IDA
Subcellular Localization
    membrane GO:0016020 ISO
    6-phosphofructokinase complex GO:0005945 ISO
    cytoplasm GO:0005737 IEA
    extracellular exosome GO:0070062 ISO
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Pfkl in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Pfkl
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Pfkl is involved
PathwayEvidenceSource
Glycolysis IEA Reactome





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