Gene description for Ppp3ca
Gene name protein phosphatase 3, catalytic subunit, alpha isoform
Gene symbol Ppp3ca
Other names/aliases 2900074D19Rik
CN
Caln
Calna
CnA
Species Mus musculus
 Database cross references - Ppp3ca
ExoCarta ExoCarta_19055
Vesiclepedia VP_19055
Entrez Gene 19055
UniProt P63328  
 Ppp3ca identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ppp3ca
Molecular Function
    protein heterodimerization activity GO:0046982 ISO
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    protein dimerization activity GO:0046983 ISO
    calmodulin-dependent protein phosphatase activity GO:0033192 ISO
    calmodulin binding GO:0005516 ISO
    protein serine/threonine phosphatase activity GO:0004722 ISO
    hydrolase activity GO:0016787 IEA
    calcium-dependent protein serine/threonine phosphatase activity GO:0004723 ISO
    enzyme binding GO:0019899 ISO
    drug binding GO:0008144 ISO
    phosphoprotein phosphatase activity GO:0004721 ISO
Biological Process
    dephosphorylation GO:0016311 IDA
    cellular response to drug GO:0035690 ISO
    negative regulation of chromatin binding GO:0035562 IMP
    protein dephosphorylation GO:0006470 ISO
    positive regulation of cardiac muscle hypertrophy in response to stress GO:1903244 IDA
    regulation of synaptic transmission GO:0050804 IMP
    positive regulation of sequence-specific DNA binding transcription factor activity GO:0051091 IDA
    cellular response to glucose stimulus GO:0071333 ISO
    calcineurin-NFAT signaling cascade GO:0033173 ISO
    regulation of excitatory postsynaptic membrane potential GO:0060079 IGI
    protein import into nucleus GO:0006606 IDA
    multicellular organismal response to stress GO:0033555 IDA
    calcium ion transport GO:0006816 IMP
    cardiac muscle hypertrophy in response to stress GO:0014898 IGI
    response to calcium ion GO:0051592 ISO
    skeletal muscle fiber development GO:0048741 IMP
    negative regulation of dendrite morphogenesis GO:0050774 IMP
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    positive regulation of NFAT protein import into nucleus GO:0051533 ISO
    G1/S transition of mitotic cell cycle GO:0000082 IMP
    calcium-mediated signaling GO:0019722 IGI
    negative regulation of insulin secretion GO:0046676 ISO
    transition between fast and slow fiber GO:0014883 IDA
Subcellular Localization
    Z disc GO:0030018 IDA
    plasma membrane GO:0005886 IEA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 ISO
    nucleus GO:0005634 IDA
    calcineurin complex GO:0005955 ISO
    nucleoplasm GO:0005654 ISO
    intracellular GO:0005622 IGI
    mitochondrion GO:0005739 IDA
    membrane GO:0016020 ISO
 Experiment description of studies that identified Ppp3ca in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ppp3ca
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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