Gene description for Wnk2
Gene name WNK lysine deficient protein kinase 2
Gene symbol Wnk2
Other names/aliases 1810073P09Rik
AW122246
ESTM15
X83337
mKIAA1760
Species Mus musculus
 Database cross references - Wnk2
ExoCarta ExoCarta_75607
Entrez Gene 75607
UniProt Q3UH66  
 Wnk2 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Wnk2
Molecular Function
    protein kinase activity GO:0004672 IEA
    protein serine/threonine kinase activity GO:0004674 IEA
    transferase activity, transferring phosphorus-containing groups GO:0016772 IEA
    kinase activity GO:0016301 IEA
    transferase activity GO:0016740 IEA
    ATP binding GO:0005524 IEA
    molecular_function GO:0003674 ND
    nucleotide binding GO:0000166 IEA
Biological Process
    negative regulation of ERK1 and ERK2 cascade GO:0070373 ISO
    protein phosphorylation GO:0006468 ISO
    protein autophosphorylation GO:0046777 ISO
    regulation of ion homeostasis GO:2000021 ISO
    positive regulation of sodium ion transmembrane transporter activity GO:2000651 ISO
    phosphorylation GO:0016310 IEA
    negative regulation of cell proliferation GO:0008285 ISO
Subcellular Localization
    plasma membrane GO:0005886 IEA
    membrane GO:0016020 IEA
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Wnk2 in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Wnk2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Wnk2 is involved
PathwayEvidenceSource
Stimuli-sensing channels IEA Reactome





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