Gene description for Wnk2
Gene name WNK lysine deficient protein kinase 2
Gene symbol Wnk2
Other names/aliases 1810073P09Rik
AW122246
ESTM15
X83337
mKIAA1760
Species Mus musculus
 Database cross references - Wnk2
ExoCarta ExoCarta_75607
Vesiclepedia VP_75607
Entrez Gene 75607
UniProt Q3UH66  
 Wnk2 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Wnk2
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IBA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    ATP binding GO:0005524 IEA
    potassium channel inhibitor activity GO:0019870 IBA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    protein phosphorylation GO:0006468 ISO
    negative regulation of cell population proliferation GO:0008285 ISO
    negative regulation of sodium ion transport GO:0010766 IBA
    intracellular signal transduction GO:0035556 IBA
    protein autophosphorylation GO:0046777 ISO
    monoatomic ion homeostasis GO:0050801 IBA
    monoatomic ion homeostasis GO:0050801 ISO
    negative regulation of ERK1 and ERK2 cascade GO:0070373 ISO
    positive regulation of canonical Wnt signaling pathway GO:0090263 ISO
    positive regulation of potassium ion import across plasma membrane GO:1903288 IBA
    positive regulation of sodium ion transmembrane transporter activity GO:2000651 ISO
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
 Experiment description of studies that identified Wnk2 in exosomes
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Wnk2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Tsc1  
Affinity Capture-MS Mus musculus
3 Rilpl1  
Co-fractionation Mus musculus
4 Klhl3  
Co-fractionation Mus musculus
5 OTUD4  
Reconstituted Complex Homo sapiens
6 Cobll1 319876
Co-fractionation Mus musculus
7 Htt  
Co-fractionation Mus musculus
8 Wnk3  
Co-fractionation Mus musculus
9 Wnk1 232341
Co-fractionation Mus musculus
10 Ywhaz 22631
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Wnk2 is involved
No pathways found





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