Gene description for Wnk1
Gene name WNK lysine deficient protein kinase 1
Gene symbol Wnk1
Other names/aliases 6430573H23Rik
EG406236
Hsn2
Prkwnk1
mKIAA0344
Species Mus musculus
 Database cross references - Wnk1
ExoCarta ExoCarta_232341
Vesiclepedia VP_232341
Entrez Gene 232341
UniProt P83741  
 Wnk1 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Wnk1
Molecular Function
    magnesium ion binding GO:0000287 ISO
    protein kinase activity GO:0004672 IDA
    protein kinase activity GO:0004672 ISO
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IMP
    protein serine/threonine kinase activity GO:0004674 ISO
    protein serine/threonine kinase activity GO:0004674 ISS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    potassium channel inhibitor activity GO:0019870 IBA
    potassium channel inhibitor activity GO:0019870 ISO
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    phosphatase binding GO:0019902 ISO
    phosphatase binding GO:0019902 ISS
    protein serine/threonine kinase inhibitor activity GO:0030291 ISO
    protein kinase activator activity GO:0030295 IEA
    protein kinase activator activity GO:0030295 ISO
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    molecular condensate scaffold activity GO:0140693 ISO
    molecular condensate scaffold activity GO:0140693 ISS
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    regulation of sodium ion transport GO:0002028 ISO
    regulation of sodium ion transport GO:0002028 ISS
    positive regulation of systemic arterial blood pressure GO:0003084 IMP
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    protein phosphorylation GO:0006468 ISO
    monoatomic ion transport GO:0006811 IDA
    cell volume homeostasis GO:0006884 ISO
    cell volume homeostasis GO:0006884 ISS
    signal transduction GO:0007165 ISO
    heart development GO:0007507 IMP
    regulation of blood pressure GO:0008217 IMP
    negative regulation of autophagy GO:0010507 ISO
    negative regulation of autophagy GO:0010507 ISS
    negative regulation of sodium ion transport GO:0010766 IBA
    negative regulation of sodium ion transport GO:0010766 ISO
    negative regulation of sodium ion transport GO:0010766 ISS
    regulation of mRNA export from nucleus GO:0010793 ISO
    regulation of mRNA export from nucleus GO:0010793 ISS
    positive regulation of T cell chemotaxis GO:0010820 IEA
    positive regulation of T cell chemotaxis GO:0010820 ISO
    peptidyl-serine phosphorylation GO:0018105 IMP
    peptidyl-threonine phosphorylation GO:0018107 IMP
    peptidyl-threonine phosphorylation GO:0018107 ISO
    intracellular chloride ion homeostasis GO:0030644 IGI
    negative regulation of protein ubiquitination GO:0031397 ISO
    negative regulation of protein ubiquitination GO:0031397 ISS
    negative regulation of cell-cell adhesion mediated by integrin GO:0033633 IEA
    negative regulation of cell-cell adhesion mediated by integrin GO:0033633 ISO
    negative regulation of heterotypic cell-cell adhesion GO:0034115 IEA
    negative regulation of heterotypic cell-cell adhesion GO:0034115 ISO
    negative regulation of GTPase activity GO:0034260 IMP
    negative regulation of GTPase activity GO:0034260 ISO
    intracellular signal transduction GO:0035556 IBA
    intracellular signal transduction GO:0035556 IEA
    intracellular signal transduction GO:0035556 ISO
    sodium ion transmembrane transport GO:0035725 IMP
    chemokine (C-C motif) ligand 21 signaling pathway GO:0038116 IMP
    protein insertion into ER membrane by stop-transfer membrane-anchor sequence GO:0045050 ISO
    protein insertion into ER membrane by stop-transfer membrane-anchor sequence GO:0045050 ISS
    positive regulation of angiogenesis GO:0045766 IMP
    positive regulation of angiogenesis GO:0045766 ISO
    monoatomic ion homeostasis GO:0050801 IBA
    modulation of chemical synaptic transmission GO:0050804 ISO
    T cell receptor signaling pathway GO:0050852 IMP
    potassium ion homeostasis GO:0055075 IMP
    monoatomic cation homeostasis GO:0055080 IDA
    cellular response to calcium ion GO:0071277 ISO
    cellular hyperosmotic response GO:0071474 ISO
    cellular hyperosmotic response GO:0071474 ISS
    negative regulation of pancreatic juice secretion GO:0090188 IMP
    positive regulation of canonical Wnt signaling pathway GO:0090263 IEA
    positive regulation of canonical Wnt signaling pathway GO:0090263 ISO
    lymphocyte migration into lymph node GO:0097022 IMP
    membraneless organelle assembly GO:0140694 ISO
    membraneless organelle assembly GO:0140694 ISS
    regulation of sodium ion transmembrane transport GO:1902305 IMP
    negative regulation of leukocyte cell-cell adhesion GO:1903038 IEA
    negative regulation of leukocyte cell-cell adhesion GO:1903038 ISO
    negative regulation of protein localization to plasma membrane GO:1903077 ISO
    negative regulation of protein localization to plasma membrane GO:1903077 ISS
    positive regulation of potassium ion import across plasma membrane GO:1903288 IBA
    positive regulation of mitotic cytokinesis GO:1903490 ISO
    positive regulation of mitotic cytokinesis GO:1903490 ISS
    regulation of monoatomic cation transmembrane transport GO:1904062 ISO
    positive regulation of termination of RNA polymerase II transcription GO:1904595 ISO
    positive regulation of termination of RNA polymerase II transcription GO:1904595 ISS
    cellular response to chemokine GO:1990869 IMP
    cellular response to chemokine GO:1990869 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISO
    membrane GO:0016020 ISO
    protein-containing complex GO:0032991 IDA
    intracellular membraneless organelle GO:0043232 ISO
    intracellular membraneless organelle GO:0043232 ISS
    mitotic spindle GO:0072686 ISO
    mitotic spindle GO:0072686 ISS
    GABA-ergic synapse GO:0098982 ISO
 Experiment description of studies that identified Wnk1 in exosomes
1
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Wnk1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rmdn3  
Co-fractionation Mus musculus
2 Rilpl1  
Co-fractionation Mus musculus
3 Slc9a1  
Co-fractionation Mus musculus
4 Klhl3  
Co-fractionation Mus musculus
5 Rap1gap2  
Co-fractionation Mus musculus
6 Wnk2 75607
Co-fractionation Mus musculus
7 Atg5  
Affinity Capture-RNA Mus musculus
8 Prkar1b  
Co-fractionation Mus musculus
9 Nedd4l 83814
Affinity Capture-Western Mus musculus
View the network image/svg+xml
 Pathways in which Wnk1 is involved
No pathways found





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