Gene description for Braf
Gene name Braf transforming gene
Gene symbol Braf
Other names/aliases 9930012E13Rik
AA120551
AA387315
AA473386
B-raf
Braf-2
Braf2
C230098H17
C87398
D6Ertd631e
Species Mus musculus
 Database cross references - Braf
ExoCarta ExoCarta_109880
Entrez Gene 109880
UniProt P28028  
 Braf identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Braf
Molecular Function
    protein heterodimerization activity GO:0046982 ISO
    transferase activity GO:0016740 IEA
    MAP kinase kinase kinase activity GO:0004709 ISO
    kinase activity GO:0016301 IEA
    identical protein binding GO:0042802 ISO
    receptor signaling protein activity GO:0005057 IEA
    mitogen-activated protein kinase kinase binding GO:0031434 ISO
    metal ion binding GO:0046872 IEA
    transferase activity, transferring phosphorus-containing groups GO:0016772 IEA
    protein serine/threonine kinase activity GO:0004674 ISO
    ATP binding GO:0005524 IEA
    nucleotide binding GO:0000166 IEA
    calcium ion binding GO:0005509 ISO
    protein kinase activity GO:0004672 ISO
    protein binding GO:0005515 IPI
Biological Process
    response to cAMP GO:0051591 ISO
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 IMP
    positive regulation of peptidyl-serine phosphorylation GO:0033138 ISO
    alpha-beta T cell differentiation GO:0046632 IMP
    positive regulation of gene expression GO:0010628 ISO
    protein heterooligomerization GO:0051291 ISO
    visual learning GO:0008542 IMP
    negative regulation of apoptotic process GO:0043066 ISO
    cellular response to drug GO:0035690 IDA
    regulation of cell proliferation GO:0042127 IGI
    protein phosphorylation GO:0006468 ISO
    long-term synaptic potentiation GO:0060291 IMP
    glucose transport GO:0015758 ISO
    MAPK cascade GO:0000165 ISO
    signal transduction GO:0007165 IEA
    negative regulation of synaptic vesicle exocytosis GO:2000301 IMP
    CD4-positive, alpha-beta T cell differentiation GO:0043367 IMP
    cell differentiation GO:0030154 IGI
    positive T cell selection GO:0043368 IMP
    negative regulation of endothelial cell apoptotic process GO:2000352 IMP
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISO
    cellular response to calcium ion GO:0071277 ISO
    response to peptide hormone GO:0043434 ISO
    positive regulation of stress fiber assembly GO:0051496 IMP
    somatic stem cell maintenance GO:0035019 IGI
    positive regulation of axonogenesis GO:0050772 IMP
    phosphorylation GO:0016310 IEA
    activation of MAPKK activity GO:0000186 ISO
    regulation of axon regeneration GO:0048679 IGI
    intracellular signal transduction GO:0035556 IEA
    head morphogenesis GO:0060323 IMP
    positive regulation of axon regeneration GO:0048680 IDA
    negative regulation of fibroblast migration GO:0010764 IMP
    establishment of protein localization to membrane GO:0090150 ISO
    negative regulation of neuron apoptotic process GO:0043524 ISO
    myeloid progenitor cell differentiation GO:0002318 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 IDA
    membrane GO:0016020 ISO
    plasma membrane GO:0005886 IDA
    cell body GO:0044297 IDA
    neuron projection GO:0043005 ISO
    nucleus GO:0005634 ISO
 Experiment description of studies that identified Braf in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Braf
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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