Gene description for Hras
Gene name Harvey rat sarcoma virus oncogene
Gene symbol Hras
Other names/aliases H-ras
Ha-ras
Harvey-ras
Hras-1
Hras1
Kras2
c-H-ras
c-Ha-ras
c-rasHa
ras
Species Mus musculus
 Database cross references - Hras
ExoCarta ExoCarta_15461
Entrez Gene 15461
UniProt Q61411  
 Hras identified in exosomes derived from the following tissue/cell type
Basophilic leukemia cells 20424270    
Embryonic fibroblasts 18494037    
 Gene ontology annotations for Hras
Molecular Function
    GTP binding GO:0005525 ISO
    nucleotide binding GO:0000166 IEA
    protein binding GO:0005515 IPI
    protein C-terminus binding GO:0008022 ISO
Biological Process
    negative regulation of GTPase activity GO:0034260 ISO
    intrinsic apoptotic signaling pathway GO:0097193 IMP
    mitotic cell cycle checkpoint GO:0007093 ISO
    negative regulation of gene expression GO:0010629 ISO
    regulation of long-term neuronal synaptic plasticity GO:0048169 IMP
    positive regulation of MAP kinase activity GO:0043406 ISO
    positive regulation of actin cytoskeleton reorganization GO:2000251 ISO
    positive regulation of ruffle assembly GO:1900029 ISO
    Ras protein signal transduction GO:0007265 TAS
    negative regulation of cell proliferation GO:0008285 ISO
    positive regulation of protein phosphorylation GO:0001934 ISO
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISO
    positive regulation of MAPK cascade GO:0043410 ISO
    positive regulation of epithelial cell proliferation GO:0050679 ISO
    endocytosis GO:0006897 IDA
    negative regulation of neuron apoptotic process GO:0043524 IGI
    cell aging GO:0007569 IDA
    positive regulation of cell migration GO:0030335 ISO
    positive regulation of Ras protein signal transduction GO:0046579 ISO
    positive regulation of DNA replication GO:0045740 ISO
    positive regulation of gene expression GO:0010628 IDA
    cell proliferation GO:0008283 IPI
    positive regulation of miRNA metabolic process GO:2000630 ISO
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    cellular senescence GO:0090398 ISO
    apoptotic process GO:0006915 ISO
    cell cycle arrest GO:0007050 ISO
    signal transduction GO:0007165 IEA
    small GTPase mediated signal transduction GO:0007264 IDA
    positive regulation of cell proliferation GO:0008284 ISO
    positive regulation of JNK cascade GO:0046330 ISO
    positive regulation of GTPase activity GO:0043547 ISO
    positive regulation of wound healing GO:0090303 ISO
    protein heterooligomerization GO:0051291 ISO
Subcellular Localization
    intracellular GO:0005622 IGI
    membrane GO:0016020 IEA
    Golgi apparatus GO:0005794 ISO
    plasma membrane GO:0005886 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified Hras in exosomes
1
Experiment ID 89
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20424270    
Organism Rattus norvegicus
Experiment description Exosomes account for vesicle-mediated transcellular transport of activatable phospholipases and prostaglandins.
Authors Subra C, Grand D, Laulagnier K, Stella A, Lambeau G, Paillasse M, De Medina P, Monsarrat B, Perret B, Silvente-Poirot S, Poirot M, Record M.
Journal name JLR
Publication year 2010
Sample Basophilic leukemia cells
Sample name RBL-2H3
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Gas chromatography
Thin layer chromatography
Mass spectrometry [Qstar XL]
FACS
2
Experiment ID 73
ISEV standards
EM
EV Biophysical techniques
HSP70|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
 Protein-protein interactions for Hras
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAF1 5894
Reconstituted Complex Homo sapiens
2 Raf1  
Affinity Capture-Western Mus musculus
View the network image/svg+xml
 Pathways in which Hras is involved
PathwayEvidenceSource
Activation of RAS in B cells IEA Reactome
CD209 (DC-SIGN) signaling IEA Reactome
DAP12 signaling IEA Reactome
Downstream signal transduction IEA Reactome
EGFR Transactivation by Gastrin IEA Reactome
EPHB-mediated forward signaling IEA Reactome
FCERI mediated MAPK activation IEA Reactome
FRS-mediated FGFR1 signaling IEA Reactome
FRS-mediated FGFR2 signaling IEA Reactome
FRS-mediated FGFR3 signaling IEA Reactome
FRS2-mediated FGFR4 signaling IEA Reactome
GRB2 events in EGFR signaling IEA Reactome
GRB2 events in ERBB2 signaling IEA Reactome
Interleukin receptor SHC signaling IEA Reactome
Interleukin receptor SHC signaling IEA Reactome
MEK activation IEA Reactome
NCAM signaling for neurite out-growth IEA Reactome
p38MAPK events IEA Reactome
RAF activation IEA Reactome
RAF phosphorylates MEK IEA Reactome
Ras activation uopn Ca2+ infux through NMDA receptor IEA Reactome
SHC-mediated cascade:FGFR1 IEA Reactome
SHC-mediated cascade:FGFR2 IEA Reactome
SHC-mediated cascade:FGFR3 IEA Reactome
SHC-mediated cascade:FGFR4 IEA Reactome
SHC-mediated signalling IEA Reactome
SHC-mediated signalling IEA Reactome
SHC1 events in EGFR signaling IEA Reactome
SHC1 events in ERBB2 signaling IEA Reactome
SHC1 events in ERBB4 signaling IEA Reactome
Signaling by SCF-KIT IEA Reactome
Signalling to RAS IEA Reactome
SOS-mediated signalling IEA Reactome
SOS-mediated signalling IEA Reactome
Tie2 Signaling IEA Reactome
VEGFR2 mediated cell proliferation IEA Reactome





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