Gene description for Hras
Gene name Harvey rat sarcoma virus oncogene
Gene symbol Hras
Other names/aliases H-ras
Ha-ras
Harvey-ras
Hras-1
Hras1
Kras2
c-H-ras
c-Ha-ras
c-rasHa
ras
Species Mus musculus
 Database cross references - Hras
ExoCarta ExoCarta_15461
Vesiclepedia VP_15461
Entrez Gene 15461
UniProt Q61411  
 Hras identified in sEVs derived from the following tissue/cell type
Basophilic leukemia cells 20424270    
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hras
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 ISO
    G protein activity GO:0003925 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IDA
    GTP binding GO:0005525 ISO
    GDP binding GO:0019003 IBA
    GDP binding GO:0019003 IEA
    GDP binding GO:0019003 ISO
    protein-membrane adaptor activity GO:0043495 IDA
    protein-membrane adaptor activity GO:0043495 ISO
    protein-containing complex binding GO:0044877 ISO
    phospholipase C activator activity GO:0160185 IEA
Biological Process
    positive regulation of protein phosphorylation GO:0001934 ISO
    regulation of transcription by RNA polymerase II GO:0006357 ISO
    endocytosis GO:0006897 IDA
    small GTPase-mediated signal transduction GO:0007264 IDA
    Ras protein signal transduction GO:0007265 IBA
    Ras protein signal transduction GO:0007265 IGI
    Ras protein signal transduction GO:0007265 ISO
    Ras protein signal transduction GO:0007265 TAS
    positive regulation of cell population proliferation GO:0008284 IPI
    positive regulation of cell population proliferation GO:0008284 ISO
    negative regulation of cell population proliferation GO:0008285 IEA
    negative regulation of cell population proliferation GO:0008285 ISO
    insulin receptor signaling pathway GO:0008286 IDA
    positive regulation of gene expression GO:0010628 IDA
    negative regulation of gene expression GO:0010629 IEA
    negative regulation of gene expression GO:0010629 ISO
    Schwann cell development GO:0014044 IMP
    positive regulation of cell migration GO:0030335 IEA
    positive regulation of cell migration GO:0030335 ISO
    positive regulation of type II interferon production GO:0032729 IMP
    regulation of actin cytoskeleton organization GO:0032956 IEA
    regulation of actin cytoskeleton organization GO:0032956 ISO
    negative regulation of GTPase activity GO:0034260 ISO
    ERBB2-ERBB3 signaling pathway GO:0038133 ISO
    T-helper 1 type immune response GO:0042088 IMP
    regulation of cell population proliferation GO:0042127 ISO
    myelination GO:0042552 IMP
    defense response to protozoan GO:0042832 IMP
    positive regulation of MAP kinase activity GO:0043406 ISO
    positive regulation of MAPK cascade GO:0043410 ISO
    negative regulation of neuron apoptotic process GO:0043524 IDA
    negative regulation of neuron apoptotic process GO:0043524 IGI
    positive regulation of GTPase activity GO:0043547 ISO
    positive regulation of DNA replication GO:0045740 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    positive regulation of JNK cascade GO:0046330 IEA
    positive regulation of JNK cascade GO:0046330 ISO
    positive regulation of Ras protein signal transduction GO:0046579 ISO
    insulin-like growth factor receptor signaling pathway GO:0048009 ISO
    fibroblast proliferation GO:0048144 IDA
    positive regulation of fibroblast proliferation GO:0048146 IDA
    regulation of long-term neuronal synaptic plasticity GO:0048169 IMP
    positive regulation of epithelial cell proliferation GO:0050679 IEA
    positive regulation of epithelial cell proliferation GO:0050679 ISO
    T cell receptor signaling pathway GO:0050852 IMP
    neuron apoptotic process GO:0051402 IDA
    neuron apoptotic process GO:0051402 IGI
    regulation of cell cycle GO:0051726 IEA
    regulation of cell cycle GO:0051726 ISO
    adipose tissue development GO:0060612 IMP
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IEA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISO
    cellular response to gamma radiation GO:0071480 IEA
    cellular response to gamma radiation GO:0071480 ISO
    positive regulation of wound healing GO:0090303 IEA
    positive regulation of wound healing GO:0090303 ISO
    positive regulation of protein targeting to membrane GO:0090314 IEA
    positive regulation of protein targeting to membrane GO:0090314 ISO
    cellular senescence GO:0090398 IBA
    cellular senescence GO:0090398 IDA
    cellular senescence GO:0090398 ISO
    oncogene-induced cell senescence GO:0090402 IDA
    intrinsic apoptotic signaling pathway GO:0097193 IGI
    intrinsic apoptotic signaling pathway GO:0097193 IMP
    regulation of neurotransmitter receptor localization to postsynaptic specialization membrane GO:0098696 IEA
    regulation of neurotransmitter receptor localization to postsynaptic specialization membrane GO:0098696 ISO
    positive regulation of ruffle assembly GO:1900029 IEA
    positive regulation of ruffle assembly GO:1900029 ISO
    positive regulation of miRNA metabolic process GO:2000630 IEA
    positive regulation of miRNA metabolic process GO:2000630 ISO
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    Golgi apparatus GO:0005794 ISO
    Golgi apparatus GO:0005794 ISS
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    glutamatergic synapse GO:0098978 IEA
    glutamatergic synapse GO:0098978 ISO
    GTPase complex GO:1905360 ISO
 Experiment description of studies that identified Hras in sEVs
1
Experiment ID 89
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20424270    
Organism Rattus norvegicus
Experiment description Exosomes account for vesicle-mediated transcellular transport of activatable phospholipases and prostaglandins.
Authors "Subra C, Grand D, Laulagnier K, Stella A, Lambeau G, Paillasse M, De Medina P, Monsarrat B, Perret B, Silvente-Poirot S, Poirot M, Record M."
Journal name JLR
Publication year 2010
Sample Basophilic leukemia cells
Sample name RBL-2H3
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Gas chromatography
Thin layer chromatography
Mass spectrometry [Qstar XL]
FACS
2
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hras
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAF1 5894
Reconstituted Complex Homo sapiens
2 Tsc1  
Affinity Capture-MS Mus musculus
3 Brap  
Affinity Capture-Western Mus musculus
4 Grin2b  
Affinity Capture-Western Mus musculus
5 Braf 109880
Two-hybrid Mus musculus
Two-hybrid Mus musculus
6 Abi1 11308
Phenotypic Suppression Mus musculus
7 Rassf5  
Affinity Capture-Western Mus musculus
8 Rin1  
Affinity Capture-Western Mus musculus
Two-hybrid Mus musculus
9 Tcf4 21413
Phenotypic Suppression Mus musculus
10 Zhx2  
Affinity Capture-Western Mus musculus
11 Fancd2  
Affinity Capture-MS Mus musculus
12 Pik3r1  
Affinity Capture-Western Mus musculus
13 Apc  
Phenotypic Suppression Mus musculus
14 Raf1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Two-hybrid Mus musculus
Two-hybrid Mus musculus
Two-hybrid Mus musculus
15 Ralgds  
Two-hybrid Mus musculus
View the network image/svg+xml
 Pathways in which Hras is involved
PathwayEvidenceSource
Activation of RAS in B cells IEA Reactome
Adaptive Immune System IEA Reactome
Axon guidance IEA Reactome
C-type lectin receptors (CLRs) IEA Reactome
CD209 (DC-SIGN) signaling IEA Reactome
Cell surface interactions at the vascular wall IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
DAP12 interactions IEA Reactome
DAP12 signaling IEA Reactome
Developmental Biology IEA Reactome
Downstream signal transduction IEA Reactome
Downstream signaling events of B Cell Receptor (BCR) IEA Reactome
Downstream signaling of activated FGFR1 IEA Reactome
Downstream signaling of activated FGFR2 IEA Reactome
Downstream signaling of activated FGFR3 IEA Reactome
Downstream signaling of activated FGFR4 IEA Reactome
EGFR Transactivation by Gastrin IEA Reactome
EPH-Ephrin signaling IEA Reactome
EPHB-mediated forward signaling IEA Reactome
ESR-mediated signaling IEA Reactome
Estrogen-stimulated signaling through PRKCZ IEA Reactome
Extra-nuclear estrogen signaling IEA Reactome
Fc epsilon receptor (FCERI) signaling IEA Reactome
FCERI mediated MAPK activation IEA Reactome
FLT3 Signaling IEA Reactome
FRS-mediated FGFR1 signaling IEA Reactome
FRS-mediated FGFR2 signaling IEA Reactome
FRS-mediated FGFR3 signaling IEA Reactome
FRS-mediated FGFR4 signaling IEA Reactome
G alpha (q) signalling events IEA Reactome
Gastrin-CREB signalling pathway via PKC and MAPK IEA Reactome
GPCR downstream signalling IEA Reactome
GRB2 events in EGFR signaling IEA Reactome
GRB2 events in ERBB2 signaling IEA Reactome
Hemostasis IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
MAP2K and MAPK activation IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK1/MAPK3 signaling IEA Reactome
MET activates RAS signaling IEA Reactome
NCAM signaling for neurite out-growth IEA Reactome
Negative regulation of MAPK pathway IEA Reactome
Nervous system development IEA Reactome
p38MAPK events IEA Reactome
PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases IEA Reactome
RAF activation IEA Reactome
RAF/MAP kinase cascade IEA Reactome
RAS processing IEA Reactome
Regulation of RAS by GAPs IEA Reactome
SHC-mediated cascade:FGFR1 IEA Reactome
SHC-mediated cascade:FGFR2 IEA Reactome
SHC-mediated cascade:FGFR3 IEA Reactome
SHC-mediated cascade:FGFR4 IEA Reactome
SHC1 events in EGFR signaling IEA Reactome
SHC1 events in ERBB4 signaling IEA Reactome
Signal Transduction IEA Reactome
Signaling by EGFR IEA Reactome
Signaling by ERBB2 IEA Reactome
Signaling by ERBB4 IEA Reactome
Signaling by FGFR IEA Reactome
Signaling by FGFR1 IEA Reactome
Signaling by FGFR2 IEA Reactome
Signaling by FGFR3 IEA Reactome
Signaling by FGFR4 IEA Reactome
Signaling by GPCR IEA Reactome
Signaling by MET IEA Reactome
Signaling by Non-Receptor Tyrosine Kinases IEA Reactome
Signaling by NTRK1 (TRKA) IEA Reactome
Signaling by NTRKs IEA Reactome
Signaling by Nuclear Receptors IEA Reactome
Signaling by PDGF IEA Reactome
Signaling by PTK6 IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by SCF-KIT IEA Reactome
Signaling by the B Cell Receptor (BCR) IEA Reactome
Signaling by VEGF IEA Reactome
Signalling to ERKs IEA Reactome
Signalling to RAS IEA Reactome
Tie2 Signaling IEA Reactome
VEGFA-VEGFR2 Pathway IEA Reactome
VEGFR2 mediated cell proliferation IEA Reactome





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