Gene description for Ppp2ca
Gene name protein phosphatase 2, catalytic subunit, alpha isozyme
Gene symbol Ppp2ca
Other names/aliases Pp2a1
Species Rattus norvegicus
 Database cross references - Ppp2ca
ExoCarta ExoCarta_24672
Vesiclepedia VP_24672
Entrez Gene 24672
UniProt P63331  
 Ppp2ca identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Ppp2ca
Molecular Function
    phosphoprotein phosphatase activity GO:0004721 IDA
    protein serine/threonine phosphatase activity GO:0004722 IBA
    protein serine/threonine phosphatase activity GO:0004722 IDA
    protein serine/threonine phosphatase activity GO:0004722 IEA
    protein serine/threonine phosphatase activity GO:0004722 IMP
    protein serine/threonine phosphatase activity GO:0004722 ISO
    protein serine/threonine phosphatase activity GO:0004722 TAS
    protein tyrosine phosphatase activity GO:0004725 ISO
    protein binding GO:0005515 IPI
    myosin phosphatase activity GO:0017018 IEA
    enzyme binding GO:0019899 IPI
    protein kinase binding GO:0019901 IDA
    protein kinase binding GO:0019901 IPI
    protein phosphatase binding GO:0019903 IPI
    protein domain specific binding GO:0019904 IDA
    beta-2 adrenergic receptor binding GO:0031698 IPI
    calmodulin-dependent protein phosphatase activity GO:0033192 IEA
    identical protein binding GO:0042802 IMP
    protein kinase B binding GO:0043422 IPI
    transmembrane transporter binding GO:0044325 IPI
    protein-containing complex binding GO:0044877 IPI
    metal ion binding GO:0046872 IEA
    protein heterodimerization activity GO:0046982 ISO
    protein heterodimerization activity GO:0046982 ISS
    tau protein binding GO:0048156 IPI
    GABA receptor binding GO:0050811 ISO
    protein phosphatase 2A binding GO:0051721 IDA
    protein phosphatase 2A binding GO:0051721 IPI
    histone H2AXS140 phosphatase activity GO:0140791 IEA
    RNA polymerase II CTD heptapeptide repeat Y1 phosphatase activity GO:0180004 IEA
    RNA polymerase II CTD heptapeptide repeat T4 phosphatase activity GO:0180005 IEA
    RNA polymerase II CTD heptapeptide repeat S2 phosphatase activity GO:0180006 IEA
    RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity GO:0180007 IEA
    RNA polymerase II CTD heptapeptide repeat S7 phosphatase activity GO:0180008 IEA
    protein antigen binding GO:1990405 IPI
    MAP kinase serine/threonine phosphatase activity GO:1990439 IEA
Biological Process
    mitotic cell cycle GO:0000278 IBA
    regulation of protein phosphorylation GO:0001932 IMP
    negative regulation of protein phosphorylation GO:0001933 IMP
    cardiac ventricle development GO:0003231 IEP
    chromatin remodeling GO:0006338 IEA
    protein dephosphorylation GO:0006470 IDA
    protein dephosphorylation GO:0006470 ISO
    mesoderm development GO:0007498 ISO
    heart development GO:0007507 IEP
    response to lead ion GO:0010288 IDA
    regulation of signaling receptor activity GO:0010469 IMP
    negative regulation of epithelial to mesenchymal transition GO:0010719 ISO
    regulation of protein autophosphorylation GO:0031952 IMP
    cellular response to insulin stimulus GO:0032869 IEP
    positive regulation of dephosphorylation GO:0035306 IMP
    negative regulation of hippo signaling GO:0035331 ISO
    negative regulation of hippo signaling GO:0035331 ISS
    peptidyl-threonine dephosphorylation GO:0035970 ISO
    regulation of protein catabolic process GO:0042176 IMP
    negative regulation of protein import into nucleus GO:0042308 IMP
    T cell homeostasis GO:0043029 ISO
    T cell homeostasis GO:0043029 ISS
    meiotic cell cycle GO:0051321 IEA
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 ISO
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 ISO
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 ISS
    cellular response to calcium ion GO:0071277 IEP
    cellular response to glucose stimulus GO:0071333 IEP
    cellular response to glucose stimulus GO:0071333 IMP
    cellular response to cytokine stimulus GO:0071345 IEP
    cellular response to ethanol GO:0071361 IEP
    cellular response to follicle-stimulating hormone stimulus GO:0071372 IEP
    cellular response to steroid hormone stimulus GO:0071383 IEP
    positive regulation of protein serine/threonine kinase activity GO:0071902 ISO
    positive regulation of NLRP3 inflammasome complex assembly GO:1900227 ISO
    positive regulation of NLRP3 inflammasome complex assembly GO:1900227 ISS
    negative regulation of calcium ion transmembrane transporter activity GO:1901020 IMP
    negative regulation of glycolytic process through fructose-6-phosphate GO:1904539 ISO
    regulation of G1/S transition of mitotic cell cycle GO:2000045 ISO
    positive regulation of extrinsic apoptotic signaling pathway GO:2001238 IMP
Subcellular Localization
    protein phosphatase type 2A complex GO:0000159 IMP
    protein phosphatase type 2A complex GO:0000159 ISO
    chromosome, centromeric region GO:0000775 IEA
    chromosome, centromeric region GO:0000775 ISO
    spindle pole GO:0000922 IEA
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 ISO
    protein serine/threonine phosphatase complex GO:0008287 ISO
    terminal bouton GO:0043195 IDA
    membrane raft GO:0045121 ISO
    membrane raft GO:0045121 ISS
    synapse GO:0045202 ISO
    FAR/SIN/STRIPAK complex GO:0090443 ISO
 Experiment description of studies that identified Ppp2ca in exosomes
1
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 101
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ppp2ca
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ppp2r1a 51792
Affinity Capture-Western Mus musculus
2 Grin3a  
Affinity Capture-Western Rattus norvegicus
3 Snca  
Affinity Capture-Western Rattus norvegicus
4 PPP2R1A 5518
Affinity Capture-Western Homo sapiens
5 TIPRL 261726
Affinity Capture-Western Homo sapiens
6 Sumo3  
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Ppp2ca is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal IEA Reactome
Amplification of signal from the kinetochores IEA Reactome
Antiviral mechanism by IFN-stimulated genes IEA Reactome
Beta-catenin phosphorylation cascade IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular responses to mechanical stimuli IEA Reactome
Cellular responses to stimuli IEA Reactome
Costimulation by the CD28 family IEA Reactome
CTLA4 inhibitory signaling IEA Reactome
Cyclin A/B1/B2 associated events during G2/M transition IEA Reactome
Cyclin D associated events in G1 IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
DARPP-32 events IEA Reactome
Degradation of beta-catenin by the destruction complex IEA Reactome
Disassembly of the destruction complex and recruitment of AXIN to the membrane IEA Reactome
E2F mediated regulation of DNA replication IEA Reactome
EML4 and NUDC in mitotic spindle formation IEA Reactome
ERK/MAPK targets IEA Reactome
ERKs are inactivated IEA Reactome
G alpha (i) signalling events IEA Reactome
G1 Phase IEA Reactome
G1/S Transition IEA Reactome
G2/M Transition IEA Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway IEA Reactome
GPCR downstream signalling IEA Reactome
Hemostasis IEA Reactome
Immune System IEA Reactome
Inhibition of replication initiation of damaged DNA by RB1/E2F1 IEA Reactome
Initiation of Nuclear Envelope (NE) Reformation IEA Reactome
Innate Immune System IEA Reactome
Interferon Signaling IEA Reactome
Interleukin-17 signaling IEA Reactome
Intracellular signaling by second messengers IEA Reactome
M Phase IEA Reactome
MAP kinase activation IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK targets/ Nuclear events mediated by MAP kinases IEA Reactome
MAPK1/MAPK3 signaling IEA Reactome
Metabolism of RNA IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic G1 phase and G1/S transition IEA Reactome
Mitotic G2-G2/M phases IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prometaphase IEA Reactome
Mitotic Spindle Checkpoint IEA Reactome
MyD88 cascade initiated on plasma membrane IEA Reactome
MyD88 dependent cascade initiated on endosome IEA Reactome
MyD88-independent TLR4 cascade IEA Reactome
MyD88:MAL(TIRAP) cascade initiated on plasma membrane IEA Reactome
Negative regulation of FGFR1 signaling IEA Reactome
Negative regulation of FGFR2 signaling IEA Reactome
Negative regulation of FGFR3 signaling IEA Reactome
Negative regulation of FGFR4 signaling IEA Reactome
Negative regulation of MAPK pathway IEA Reactome
Negative regulation of the PI3K/AKT network IEA Reactome
Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) IEA Reactome
Nonsense-Mediated Decay (NMD) IEA Reactome
Nuclear Envelope (NE) Reassembly IEA Reactome
Nuclear Events (kinase and transcription factor activation) IEA Reactome
Opioid Signalling IEA Reactome
PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling IEA Reactome
PIP3 activates AKT signaling IEA Reactome
PKR-mediated signaling IEA Reactome
Platelet homeostasis IEA Reactome
Platelet sensitization by LDL IEA Reactome
RAF activation IEA Reactome
RAF/MAP kinase cascade IEA Reactome
Regulation of TP53 Activity IEA Reactome
Regulation of TP53 Degradation IEA Reactome
Regulation of TP53 Expression and Degradation IEA Reactome
Resolution of Sister Chromatid Cohesion IEA Reactome
Response of endothelial cells to shear stress IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate Formins IEA Reactome
RNA Polymerase II Transcription IEA Reactome
Separation of Sister Chromatids IEA Reactome
Signal Transduction IEA Reactome
Signaling by FGFR IEA Reactome
Signaling by FGFR1 IEA Reactome
Signaling by FGFR2 IEA Reactome
Signaling by FGFR3 IEA Reactome
Signaling by FGFR4 IEA Reactome
Signaling by GPCR IEA Reactome
Signaling by Interleukins IEA Reactome
Signaling by NTRK1 (TRKA) IEA Reactome
Signaling by NTRKs IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by WNT IEA Reactome
Spry regulation of FGF signaling IEA Reactome
TCF dependent signaling in response to WNT IEA Reactome
Toll Like Receptor 10 (TLR10) Cascade IEA Reactome
Toll Like Receptor 2 (TLR2) Cascade IEA Reactome
Toll Like Receptor 3 (TLR3) Cascade IEA Reactome
Toll Like Receptor 4 (TLR4) Cascade IEA Reactome
Toll Like Receptor 5 (TLR5) Cascade IEA Reactome
Toll Like Receptor 7/8 (TLR7/8) Cascade IEA Reactome
Toll Like Receptor 9 (TLR9) Cascade IEA Reactome
Toll Like Receptor TLR1:TLR2 Cascade IEA Reactome
Toll Like Receptor TLR6:TLR2 Cascade IEA Reactome
Toll-like Receptor Cascades IEA Reactome
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation IEA Reactome
Transcriptional Regulation by TP53 IEA Reactome
TRIF (TICAM1)-mediated TLR4 signaling IEA Reactome
Turbulent (oscillatory, disturbed) flow shear stress activates signaling by PIEZO1 and integrins in endothelial cells IEA Reactome





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