Gene description for Ppp2ca
Gene name protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
Gene symbol Ppp2ca
Other names/aliases PP2A
R75353
Species Mus musculus
 Database cross references - Ppp2ca
ExoCarta ExoCarta_19052
Vesiclepedia VP_19052
Entrez Gene 19052
UniProt P63330  
 Ppp2ca identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ppp2ca
Molecular Function
    protein serine/threonine phosphatase activity GO:0004722 IBA
    protein serine/threonine phosphatase activity GO:0004722 IMP
    protein serine/threonine phosphatase activity GO:0004722 ISO
    protein serine/threonine phosphatase activity GO:0004722 ISS
    protein serine/threonine phosphatase activity GO:0004722 TAS
    protein tyrosine phosphatase activity GO:0004725 IEA
    protein tyrosine phosphatase activity GO:0004725 ISO
    protein binding GO:0005515 IPI
    myosin phosphatase activity GO:0017018 IEA
    enzyme binding GO:0019899 ISO
    protein kinase binding GO:0019901 ISO
    protein phosphatase binding GO:0019903 ISO
    protein domain specific binding GO:0019904 ISO
    beta-2 adrenergic receptor binding GO:0031698 ISO
    calmodulin-dependent protein phosphatase activity GO:0033192 IEA
    identical protein binding GO:0042802 ISO
    protein kinase B binding GO:0043422 ISO
    transmembrane transporter binding GO:0044325 ISO
    protein-containing complex binding GO:0044877 ISO
    metal ion binding GO:0046872 IEA
    protein heterodimerization activity GO:0046982 ISO
    protein heterodimerization activity GO:0046982 ISS
    tau protein binding GO:0048156 ISO
    GABA receptor binding GO:0050811 IDA
    protein phosphatase 2A binding GO:0051721 ISO
    histone H2AXS140 phosphatase activity GO:0140791 IEA
    RNA polymerase II CTD heptapeptide repeat Y1 phosphatase activity GO:0180004 IEA
    RNA polymerase II CTD heptapeptide repeat T4 phosphatase activity GO:0180005 IEA
    RNA polymerase II CTD heptapeptide repeat S2 phosphatase activity GO:0180006 IEA
    RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity GO:0180007 IEA
    RNA polymerase II CTD heptapeptide repeat S7 phosphatase activity GO:0180008 IEA
    protein antigen binding GO:1990405 ISO
    MAP kinase serine/threonine phosphatase activity GO:1990439 IEA
Biological Process
    mitotic cell cycle GO:0000278 IBA
    regulation of protein phosphorylation GO:0001932 ISO
    chromatin remodeling GO:0006338 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    protein dephosphorylation GO:0006470 ISO
    protein dephosphorylation GO:0006470 ISS
    protein dephosphorylation GO:0006470 TAS
    mesoderm development GO:0007498 IMP
    regulation of signaling receptor activity GO:0010469 ISO
    negative regulation of epithelial to mesenchymal transition GO:0010719 IEA
    negative regulation of epithelial to mesenchymal transition GO:0010719 ISO
    regulation of protein autophosphorylation GO:0031952 ISO
    negative regulation of hippo signaling GO:0035331 ISO
    negative regulation of hippo signaling GO:0035331 ISS
    peptidyl-threonine dephosphorylation GO:0035970 ISO
    regulation of protein catabolic process GO:0042176 ISO
    T cell homeostasis GO:0043029 IMP
    meiotic cell cycle GO:0051321 IEA
    regulation of cell cycle GO:0051726 TAS
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 IMP
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 ISO
    positive regulation of protein serine/threonine kinase activity GO:0071902 ISO
    positive regulation of NLRP3 inflammasome complex assembly GO:1900227 IMP
    negative regulation of glycolytic process through fructose-6-phosphate GO:1904539 IMP
    positive regulation of glycolytic process through fructose-6-phosphate GO:1904540 ISO
    regulation of G1/S transition of mitotic cell cycle GO:2000045 IEA
    regulation of G1/S transition of mitotic cell cycle GO:2000045 ISO
Subcellular Localization
    protein phosphatase type 2A complex GO:0000159 IDA
    protein phosphatase type 2A complex GO:0000159 ISO
    chromosome, centromeric region GO:0000775 IDA
    spindle pole GO:0000922 IEA
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    protein serine/threonine phosphatase complex GO:0008287 ISO
    terminal bouton GO:0043195 ISO
    membrane raft GO:0045121 ISO
    membrane raft GO:0045121 ISS
    synapse GO:0045202 IDA
    synapse GO:0045202 IEP
    FAR/SIN/STRIPAK complex GO:0090443 IEA
    FAR/SIN/STRIPAK complex GO:0090443 ISO
 Experiment description of studies that identified Ppp2ca in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ppp2ca
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IGBP1 3476
Affinity Capture-MS Homo sapiens
2 Ppp2r1a 51792
Two-hybrid Mus musculus
3 PRICKLE3  
Affinity Capture-MS Homo sapiens
4 TRIM5 85363
Affinity Capture-MS Homo sapiens
5 MRPS7 51081
Affinity Capture-MS Homo sapiens
6 CPSF3L 54973
Affinity Capture-MS Homo sapiens
7 Akt1  
Affinity Capture-Western Mus musculus
8 SSR3 6747
Affinity Capture-MS Homo sapiens
9 Kctd20  
Reconstituted Complex Mus musculus
10 CCT8 10694
Affinity Capture-MS Homo sapiens
11 Ppfia3  
Co-fractionation Mus musculus
12 CCT3 7203
Affinity Capture-MS Homo sapiens
13 Bcl2  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
14 ERI1  
Affinity Capture-MS Homo sapiens
15 CDC73  
Affinity Capture-MS Homo sapiens
16 SIKE1  
Affinity Capture-MS Homo sapiens
17 Axin1  
Two-hybrid Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
18 TFB2M  
Affinity Capture-MS Homo sapiens
19 CCT2 10576
Affinity Capture-MS Homo sapiens
20 SENP6  
Affinity Capture-MS Homo sapiens
21 SS18L2  
Affinity Capture-MS Homo sapiens
22 Unc5b 107449
Affinity Capture-Western Mus musculus
23 TMEM109 79073
Affinity Capture-MS Homo sapiens
24 STRIP1 85369
Affinity Capture-MS Homo sapiens
25 Stk24 223255
Co-fractionation Mus musculus
26 KEAP1 9817
Affinity Capture-MS Homo sapiens
27 TCP1 6950
Affinity Capture-MS Homo sapiens
28 C1orf131  
Affinity Capture-MS Homo sapiens
29 POP4 10775
Affinity Capture-MS Homo sapiens
30 PPFIA1 8500
Affinity Capture-MS Homo sapiens
31 STRN3 29966
Affinity Capture-MS Homo sapiens
32 ZCCHC9  
Affinity Capture-MS Homo sapiens
33 CCT4 10575
Affinity Capture-MS Homo sapiens
34 INTS4  
Affinity Capture-MS Homo sapiens
35 CCDC6 8030
Affinity Capture-MS Homo sapiens
36 MOB4 25843
Affinity Capture-MS Homo sapiens
37 Rpgrip1l  
Affinity Capture-MS Mus musculus
38 STK26 51765
Affinity Capture-MS Homo sapiens
39 Strn3  
Co-fractionation Mus musculus
40 USP6NL 9712
Affinity Capture-MS Homo sapiens
41 Igbp1  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
42 SLMAP 7871
Affinity Capture-MS Homo sapiens
43 Ern2  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
44 Adcy8  
Affinity Capture-Western Mus musculus
45 CCT7 10574
Affinity Capture-MS Homo sapiens
46 CCT5 22948
Affinity Capture-MS Homo sapiens
47 Arrb2  
Reconstituted Complex Mus musculus
48 FANCM 57697
Affinity Capture-MS Homo sapiens
49 Grin2b  
Affinity Capture-Western Mus musculus
50 ATP5J2 9551
Affinity Capture-MS Homo sapiens
51 CCT6A 908
Affinity Capture-MS Homo sapiens
52 PPP2R1B 5519
Affinity Capture-MS Homo sapiens
53 STRN4 29888
Affinity Capture-MS Homo sapiens
54 INTS1 26173
Affinity Capture-MS Homo sapiens
55 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
56 FGFR1OP2  
Affinity Capture-MS Homo sapiens
57 NLRP3  
Affinity Capture-Western Homo sapiens
58 Unk  
Affinity Capture-RNA Mus musculus
59 PPP2R5D 5528
Affinity Capture-MS Homo sapiens
60 ING5  
Affinity Capture-MS Homo sapiens
61 CTTNBP2NL  
Affinity Capture-MS Homo sapiens
62 Grin1  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
63 LSM11  
Affinity Capture-MS Homo sapiens
64 Tnpo1 238799
Co-fractionation Mus musculus
65 Hist1h1a  
Biochemical Activity Mus musculus
66 Rnf2  
Affinity Capture-MS Mus musculus
67 Hnrnpa2b1 53379
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
68 ZFP91 80829
Affinity Capture-MS Homo sapiens
69 CIC 23152
Affinity Capture-MS Homo sapiens
70 Ppp2r3a  
Affinity Capture-Western Mus musculus
71 ZMYND11 10771
Affinity Capture-MS Homo sapiens
72 INTS12  
Affinity Capture-MS Homo sapiens
73 STRN 6801
Affinity Capture-MS Homo sapiens
74 MRPL22  
Affinity Capture-MS Homo sapiens
75 Mapt  
Affinity Capture-MS Mus musculus
76 Nr1i3  
Affinity Capture-Western Mus musculus
77 Nr3c1  
Affinity Capture-Western Mus musculus
78 HELLS 3070
Affinity Capture-MS Homo sapiens
79 Hdac4  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
80 BMPR1B 658
Affinity Capture-Luminescence Homo sapiens
81 PPP4R1 9989
Affinity Capture-MS Homo sapiens
82 Fancd2  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
83 Tiprl  
Co-fractionation Mus musculus
84 Ppp2r4 110854
Affinity Capture-Western Mus musculus
85 INTS6 26512
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which Ppp2ca is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal IEA Reactome
Amplification of signal from the kinetochores IEA Reactome
Antiviral mechanism by IFN-stimulated genes IEA Reactome
Beta-catenin phosphorylation cascade IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular responses to mechanical stimuli IEA Reactome
Cellular responses to stimuli IEA Reactome
Costimulation by the CD28 family IEA Reactome
CTLA4 inhibitory signaling IEA Reactome
Cyclin A/B1/B2 associated events during G2/M transition IEA Reactome
Cyclin D associated events in G1 IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
DARPP-32 events IEA Reactome
Degradation of beta-catenin by the destruction complex IEA Reactome
Disassembly of the destruction complex and recruitment of AXIN to the membrane IEA Reactome
E2F mediated regulation of DNA replication IEA Reactome
EML4 and NUDC in mitotic spindle formation IEA Reactome
ERK/MAPK targets IEA Reactome
ERKs are inactivated IEA Reactome
G alpha (i) signalling events IEA Reactome
G1 Phase IEA Reactome
G1/S Transition IEA Reactome
G2/M Transition IEA Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway IEA Reactome
GPCR downstream signalling IEA Reactome
Hemostasis IEA Reactome
Immune System IEA Reactome
Inhibition of replication initiation of damaged DNA by RB1/E2F1 IEA Reactome
Initiation of Nuclear Envelope (NE) Reformation IEA Reactome
Innate Immune System IEA Reactome
Interferon Signaling IEA Reactome
Interleukin-17 signaling IEA Reactome
Intracellular signaling by second messengers IEA Reactome
M Phase IEA Reactome
MAP kinase activation IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK targets/ Nuclear events mediated by MAP kinases IEA Reactome
MAPK1/MAPK3 signaling IEA Reactome
Metabolism of RNA IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic G1 phase and G1/S transition IEA Reactome
Mitotic G2-G2/M phases IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prometaphase IEA Reactome
Mitotic Spindle Checkpoint IEA Reactome
MyD88 cascade initiated on plasma membrane IEA Reactome
MyD88 dependent cascade initiated on endosome IEA Reactome
MyD88-independent TLR4 cascade IEA Reactome
MyD88:MAL(TIRAP) cascade initiated on plasma membrane IEA Reactome
Negative regulation of FGFR1 signaling IEA Reactome
Negative regulation of FGFR2 signaling IEA Reactome
Negative regulation of FGFR3 signaling IEA Reactome
Negative regulation of FGFR4 signaling IEA Reactome
Negative regulation of MAPK pathway IEA Reactome
Negative regulation of the PI3K/AKT network IEA Reactome
Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) IEA Reactome
Nonsense-Mediated Decay (NMD) IEA Reactome
Nuclear Envelope (NE) Reassembly IEA Reactome
Nuclear Events (kinase and transcription factor activation) IEA Reactome
Opioid Signalling IEA Reactome
PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling IEA Reactome
PIP3 activates AKT signaling IEA Reactome
PKR-mediated signaling IEA Reactome
Platelet homeostasis IEA Reactome
Platelet sensitization by LDL IEA Reactome
RAF activation IEA Reactome
RAF/MAP kinase cascade IEA Reactome
Regulation of TP53 Activity IEA Reactome
Regulation of TP53 Degradation IEA Reactome
Regulation of TP53 Expression and Degradation IEA Reactome
Resolution of Sister Chromatid Cohesion IEA Reactome
Response of endothelial cells to shear stress IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate Formins IEA Reactome
RNA Polymerase II Transcription IEA Reactome
Separation of Sister Chromatids IEA Reactome
Signal Transduction IEA Reactome
Signaling by FGFR IEA Reactome
Signaling by FGFR1 IEA Reactome
Signaling by FGFR2 IEA Reactome
Signaling by FGFR3 IEA Reactome
Signaling by FGFR4 IEA Reactome
Signaling by GPCR IEA Reactome
Signaling by Interleukins IEA Reactome
Signaling by NTRK1 (TRKA) IEA Reactome
Signaling by NTRKs IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by WNT IEA Reactome
Spry regulation of FGF signaling IEA Reactome
TCF dependent signaling in response to WNT IEA Reactome
Toll Like Receptor 10 (TLR10) Cascade IEA Reactome
Toll Like Receptor 2 (TLR2) Cascade IEA Reactome
Toll Like Receptor 3 (TLR3) Cascade IEA Reactome
Toll Like Receptor 4 (TLR4) Cascade IEA Reactome
Toll Like Receptor 5 (TLR5) Cascade IEA Reactome
Toll Like Receptor 7/8 (TLR7/8) Cascade IEA Reactome
Toll Like Receptor 9 (TLR9) Cascade IEA Reactome
Toll Like Receptor TLR1:TLR2 Cascade IEA Reactome
Toll Like Receptor TLR6:TLR2 Cascade IEA Reactome
Toll-like Receptor Cascades IEA Reactome
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation IEA Reactome
Transcriptional Regulation by TP53 IEA Reactome
TRIF (TICAM1)-mediated TLR4 signaling IEA Reactome
Turbulent (oscillatory, disturbed) flow shear stress activates signaling by PIEZO1 and integrins in endothelial cells IEA Reactome





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