Gene description for Zfand6
Gene name zinc finger, AN1-type domain 6
Gene symbol Zfand6
Other names/aliases 3110005P07Rik
Awp1
Za20d3
Species Mus musculus
 Database cross references - Zfand6
ExoCarta ExoCarta_65098
Vesiclepedia VP_65098
Entrez Gene 65098
UniProt Q9DCH6  
 Zfand6 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Zfand6
Molecular Function
    DNA binding GO:0003677 IEA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    polyubiquitin modification-dependent protein binding GO:0031593 IBA
    polyubiquitin modification-dependent protein binding GO:0031593 IDA
Biological Process
    protein targeting to peroxisome GO:0006625 IBA
    protein targeting to peroxisome GO:0006625 IDA
    apoptotic process GO:0006915 IEA
    signal transduction GO:0007165 IPI
    protein transport GO:0015031 IEA
    negative regulation of apoptotic process GO:0043066 ISO
    negative regulation of apoptotic process GO:0043066 ISS
    regulation of canonical NF-kappaB signal transduction GO:0043122 ISO
    regulation of canonical NF-kappaB signal transduction GO:0043122 ISS
    cellular response to tumor necrosis factor GO:0071356 ISO
    cellular response to tumor necrosis factor GO:0071356 ISS
Subcellular Localization
    cytoplasm GO:0005737 IEA
 Experiment description of studies that identified Zfand6 in exosomes
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Zfand6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ppp2r1a 51792
Co-fractionation Mus musculus
2 Dnm2 13430
Co-fractionation Mus musculus
3 Pkn1  
Affinity Capture-Western Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
4 Ubc  
Reconstituted Complex Mus musculus
5 Tmem173  
Proximity Label-MS Mus musculus
View the network image/svg+xml
 Pathways in which Zfand6 is involved
PathwayEvidenceSource
Peroxisomal protein import IEA Reactome
Protein localization IEA Reactome





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