Gene description for VIL1
Gene name villin 1
Gene symbol VIL1
Other names/aliases D2S1471
VIL
Species Homo sapiens
 Database cross references - VIL1
ExoCarta ExoCarta_7429
Entrez Gene 7429
HGNC 12690
MIM 193040
UniProt P09327  
 VIL1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 17956143    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 25890246    
Mesenchymal stem cells Unpublished / Not applicable
Urine 19056867    
 Gene ontology annotations for VIL1
Molecular Function
    protein binding GO:0005515 IPI
    protein homodimerization activity GO:0042803 IDA
    actin filament binding GO:0051015 IDA
    cysteine-type endopeptidase inhibitor activity involved in apoptotic process GO:0043027 ISS
    lysophosphatidic acid binding GO:0035727 IDA
    calcium ion binding GO:0005509 IMP
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IDA
    identical protein binding GO:0042802 IPI
Biological Process
    cellular response to hepatocyte growth factor stimulus GO:0035729 IEA
    cellular response to epidermal growth factor stimulus GO:0071364 IDA
    positive regulation of lamellipodium morphogenesis GO:2000394 IMP
    actin nucleation GO:0045010 IEA
    regulation of lamellipodium morphogenesis GO:2000392 ISS
    regulation of cell shape GO:0008360 IDA
    actin filament severing GO:0051014 IDA
    cytoplasmic actin-based contraction involved in cell motility GO:0060327 IDA
    actin filament polymerization GO:0030041 IDA
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 IEA
    positive regulation of cell migration GO:0030335 IDA
    epidermal growth factor receptor signaling pathway GO:0007173 IDA
    response to bacterium GO:0009617 IDA
    positive regulation of actin filament bundle assembly GO:0032233 IDA
    regulation of actin nucleation GO:0051125 IDA
    actin filament depolymerization GO:0030042 IDA
    actin filament capping GO:0051693 IDA
    barbed-end actin filament capping GO:0051016 IMP
    regulation of wound healing GO:0061041 ISS
    protein complex assembly GO:0006461 TAS
    epithelial cell differentiation GO:0030855 IEP
    positive regulation of actin filament depolymerization GO:0030836 IMP
    positive regulation of epithelial cell migration GO:0010634 IMP
Subcellular Localization
    cytoplasm GO:0005737 IEA
    brush border GO:0005903 ISS
    actin filament bundle GO:0032432 IDA
    microvillus GO:0005902 IDA
    ruffle GO:0001726 IDA
    extracellular exosome GO:0070062 IDA
    filopodium GO:0030175 IDA
    filopodium tip GO:0032433 ISS
    lamellipodium GO:0030027 IDA
 Experiment description of studies that identified VIL1 in exosomes
1
Experiment ID 20
ISEV standards
EM
EV Biophysical techniques
HSP90
EV Cytosolic markers
CD63|CD81|LAMP1
EV Membrane markers
GOLGA2|cytochrome c
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
2
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 286
ISEV standards
CEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81|EpCAM
EV Membrane markers
EV Negative markers
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
6
Experiment ID 126
ISEV standards
EV Biophysical techniques
GAPDH
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
7
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for VIL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TEX11  
Two-hybrid Homo sapiens
2 PPM1B 5495
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which VIL1 is involved
No pathways found





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