Gene description for AGPAT5
Gene name 1-acylglycerol-3-phosphate O-acyltransferase 5
Gene symbol AGPAT5
Other names/aliases 1AGPAT5
LPAATE
Species Homo sapiens
 Database cross references - AGPAT5
ExoCarta ExoCarta_55326
Entrez Gene 55326
HGNC 20886
MIM 614796
UniProt Q9NUQ2  
 AGPAT5 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for AGPAT5
Molecular Function
    protein binding GO:0005515 IPI
    1-acylglycerol-3-phosphate O-acyltransferase activity GO:0003841 NAS
Biological Process
    small molecule metabolic process GO:0044281 TAS
    glycerophospholipid biosynthetic process GO:0046474 TAS
    cellular lipid metabolic process GO:0044255 TAS
    hematopoietic progenitor cell differentiation GO:0002244 IEA
    phospholipid metabolic process GO:0006644 TAS
    triglyceride biosynthetic process GO:0019432 TAS
    phospholipid biosynthetic process GO:0008654 NAS
    phosphatidic acid biosynthetic process GO:0006654 TAS
    CDP-diacylglycerol biosynthetic process GO:0016024 IEA
Subcellular Localization
    integral component of membrane GO:0016021 IEA
    nuclear envelope GO:0005635 IEA
    mitochondrial outer membrane GO:0005741 TAS
    endoplasmic reticulum membrane GO:0005789 TAS
    mitochondrion GO:0005739 IDA
 Experiment description of studies that identified AGPAT5 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for AGPAT5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which AGPAT5 is involved
PathwayEvidenceSource
Synthesis of PA TAS Reactome
Triglyceride Biosynthesis TAS Reactome





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