Gene description for PELO
Gene name pelota homolog (Drosophila)
Gene symbol PELO
Other names/aliases CGI-17
PRO1770
Species Homo sapiens
 Database cross references - PELO
ExoCarta ExoCarta_53918
Vesiclepedia VP_53918
Entrez Gene 53918
HGNC 8829
MIM 605757
UniProt Q9BRX2  
 PELO identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for PELO
Molecular Function
    protein binding GO:0005515 IPI
    ribosome binding GO:0043022 IDA
    metal ion binding GO:0046872 IEA
    nucleoside-triphosphatase regulator activity GO:0060589 IDA
    stalled ribosome sensor activity GO:0170011 IDA
Biological Process
    inner cell mass cell proliferation GO:0001833 IEA
    regulation of translation GO:0006417 IEA
    endoderm development GO:0007492 IEA
    stem cell population maintenance GO:0019827 IEA
    positive regulation of BMP signaling pathway GO:0030513 IEA
    ribosome disassembly GO:0032790 IBA
    ribosome disassembly GO:0032790 IDA
    chromosome organization GO:0051276 IEA
    cell division GO:0051301 IEA
    mesenchymal to epithelial transition GO:0060231 IEA
    nuclear-transcribed mRNA catabolic process, non-stop decay GO:0070481 IEA
    nonfunctional rRNA decay GO:0070651 IBA
    nuclear-transcribed mRNA catabolic process, no-go decay GO:0070966 IBA
    nuclear-transcribed mRNA catabolic process, no-go decay GO:0070966 IDA
    RNA surveillance GO:0071025 IEA
    rescue of stalled ribosome GO:0072344 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosolic ribosome GO:0022626 IDA
    Dom34-Hbs1 complex GO:1990533 IDA
 Experiment description of studies that identified PELO in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PELO
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 CLPP 8192
Proximity Label-MS Homo sapiens
3 MCM7 4176
Affinity Capture-MS Homo sapiens
4 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
5 FASN 2194
Positive Genetic Homo sapiens
6 SPRTN  
Affinity Capture-MS Homo sapiens
7 PARP1 142
Proximity Label-MS Homo sapiens
8 FLNA 2316
Two-hybrid Homo sapiens
9 LRRC20  
Cross-Linking-MS (XL-MS) Homo sapiens
10 PAXIP1  
Affinity Capture-MS Homo sapiens
11 DYNLT1 6993
Two-hybrid Homo sapiens
12 EGFR 1956
Affinity Capture-MS Homo sapiens
Negative Genetic Homo sapiens
13 TP53BP1 7158
Affinity Capture-MS Homo sapiens
14 HSPA8 3312
Affinity Capture-MS Homo sapiens
15 XPO1 7514
Affinity Capture-MS Homo sapiens
16 ARRDC4  
Affinity Capture-MS Homo sapiens
17 AMBRA1  
Affinity Capture-MS Homo sapiens
18 ECT2 1894
Affinity Capture-MS Homo sapiens
19 COPG2 26958
Cross-Linking-MS (XL-MS) Homo sapiens
20 SMYD2 56950
Affinity Capture-MS Homo sapiens
21 FAS 355
Proximity Label-MS Homo sapiens
22 TIMM50 92609
Affinity Capture-MS Homo sapiens
23 TRIM66  
Affinity Capture-MS Homo sapiens
24 NOP56 10528
Co-fractionation Homo sapiens
25 ZKSCAN1  
Affinity Capture-MS Homo sapiens
26 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 TRIM33 51592
Affinity Capture-MS Homo sapiens
28 LMO4  
Two-hybrid Homo sapiens
29 SLC25A1 6576
Affinity Capture-MS Homo sapiens
30 HSPB1 3315
Two-hybrid Homo sapiens
31 TRIM55  
Two-hybrid Homo sapiens
32 PARK2  
Affinity Capture-MS Homo sapiens
33 HIST1H4A 8359
Cross-Linking-MS (XL-MS) Homo sapiens
34 DDX58 23586
Affinity Capture-RNA Homo sapiens
35 RIPK4  
Affinity Capture-MS Homo sapiens
36 HACD3 51495
Affinity Capture-MS Homo sapiens
37 HBS1L 10767
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
38 SEPT9 10801
Proximity Label-MS Homo sapiens
39 PRMT6  
Two-hybrid Homo sapiens
40 SMARCA4 6597
Co-fractionation Homo sapiens
41 PCBP1 5093
Affinity Capture-MS Homo sapiens
42 RUVBL1 8607
Affinity Capture-MS Homo sapiens
43 MYC  
Affinity Capture-MS Homo sapiens
44 C9orf72  
Affinity Capture-MS Homo sapiens
45 AIFM1 9131
Affinity Capture-MS Homo sapiens
46 KRTAP12-2  
Two-hybrid Homo sapiens
47 C16orf72 29035
Affinity Capture-MS Homo sapiens
48 YBX3 8531
Co-fractionation Homo sapiens
49 CDK9 1025
Affinity Capture-MS Homo sapiens
50 IFI16 3428
Affinity Capture-MS Homo sapiens
51 DNAJB11 51726
Affinity Capture-MS Homo sapiens
52 L3MBTL1  
Affinity Capture-MS Homo sapiens
53 BRD1 23774
Affinity Capture-MS Homo sapiens
54 MEOX2  
Two-hybrid Homo sapiens
55 LDLR 3949
Positive Genetic Homo sapiens
56 FBXL6  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
57 CAP1 10487
Cross-Linking-MS (XL-MS) Homo sapiens
58 KRAS 3845
Negative Genetic Homo sapiens
59 MDC1  
Affinity Capture-MS Homo sapiens
60 ITGA4 3676
Cross-Linking-MS (XL-MS) Homo sapiens
61 ABCD3 5825
Affinity Capture-MS Homo sapiens
62 POC1B 282809
Affinity Capture-MS Homo sapiens
63 PKM 5315
Cross-Linking-MS (XL-MS) Homo sapiens
64 SREBF2 6721
Positive Genetic Homo sapiens
65 HEATR1 55127
Affinity Capture-MS Homo sapiens
66 MYO1D 4642
Affinity Capture-MS Homo sapiens
67 SLC25A12 8604
Affinity Capture-MS Homo sapiens
68 HSP90B1 7184
Cross-Linking-MS (XL-MS) Homo sapiens
69 Ksr1  
Affinity Capture-MS Mus musculus
70 HUWE1 10075
Affinity Capture-MS Homo sapiens
71 FANCI 55215
Affinity Capture-MS Homo sapiens
72 TRIM63  
Two-hybrid Homo sapiens
73 EP300 2033
Affinity Capture-MS Homo sapiens
74 CDC5L 988
Affinity Capture-MS Homo sapiens
75 HIF1A 3091
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PELO is involved
No pathways found





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