Gene description for MAPK10
Gene name mitogen-activated protein kinase 10
Gene symbol MAPK10
Other names/aliases JNK3
JNK3A
PRKM10
SAPK1b
p493F12
p54bSAPK
Species Homo sapiens
 Database cross references - MAPK10
ExoCarta ExoCarta_5602
Vesiclepedia VP_5602
Entrez Gene 5602
HGNC 6872
MIM 602897
UniProt P53779  
 MAPK10 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for MAPK10
Molecular Function
    JUN kinase activity GO:0004705 IBA
    JUN kinase activity GO:0004705 ISS
    JUN kinase activity GO:0004705 TAS
    MAP kinase kinase activity GO:0004708 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    protein serine kinase activity GO:0106310 IEA
Biological Process
    protein phosphorylation GO:0006468 IMP
    protein phosphorylation GO:0006468 ISS
    signal transduction GO:0007165 TAS
    JNK cascade GO:0007254 IBA
    JNK cascade GO:0007254 ISS
    response to light stimulus GO:0009416 ISS
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    regulation of circadian rhythm GO:0042752 ISS
    rhythmic process GO:0048511 IEA
    cellular senescence GO:0090398 TAS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISS
    mitochondrion GO:0005739 ISS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 ISS
 Experiment description of studies that identified MAPK10 in exosomes
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MAPK10
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LDHA 3939
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
2 PKMYT1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
3 TNIP1 10318
Two-hybrid Homo sapiens
4 DUSP1  
Affinity Capture-Western Homo sapiens
5 CEP290  
Two-hybrid Homo sapiens
6 TP53 7157
Affinity Capture-Western Homo sapiens
7 DUSP10 11221
Affinity Capture-Western Homo sapiens
8 DDI1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 MAP2K4 6416
Biochemical Activity Homo sapiens
10 MAPK8IP2  
Reconstituted Complex Homo sapiens
11 CCT2 10576
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 RELA 5970
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
13 Trp53  
Biochemical Activity Mus musculus
14 GTF3C1  
Two-hybrid Homo sapiens
15 PLIN2 123
Two-hybrid Homo sapiens
16 HDAC9  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
17 HRAS 3265
Reconstituted Complex Homo sapiens
18 WDR62  
Affinity Capture-MS Homo sapiens
19 DUSP16  
Affinity Capture-Western Homo sapiens
20 HDAC1 3065
Affinity Capture-Western Homo sapiens
21 MCL1 4170
Biochemical Activity Homo sapiens
22 ARRB2 409
Affinity Capture-Western Homo sapiens
23 CCT6B 10693
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 APP 351
Reconstituted Complex Homo sapiens
25 CCT7 10574
Affinity Capture-MS Homo sapiens
26 MAPK10 5602
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
27 CREBBP  
Two-hybrid Homo sapiens
28 ATF2  
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
29 JUN 3725
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
30 ELK1  
Biochemical Activity Homo sapiens
31 SH3BP5 9467
Reconstituted Complex Homo sapiens
32 DDX5 1655
Affinity Capture-MS Homo sapiens
33 ARRB1 408
Affinity Capture-Western Homo sapiens
34 MAPK9 5601
Affinity Capture-MS Homo sapiens
35 RDX 5962
Affinity Capture-MS Homo sapiens
36 MAPK8IP3  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
37 IMPDH2 3615
Affinity Capture-MS Homo sapiens
38 UNC119 9094
Two-hybrid Homo sapiens
39 HAX1  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
40 MSN 4478
Affinity Capture-MS Homo sapiens
41 CDKN1B 1027
Reconstituted Complex Homo sapiens
42 KIF26A 26153
Two-hybrid Homo sapiens
43 EZR 7430
Affinity Capture-MS Homo sapiens
44 Rara  
Reconstituted Complex Mus musculus
View the network image/svg+xml
 Pathways in which MAPK10 is involved
PathwayEvidenceSource
Activation of the AP-1 family of transcription factors TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Cellular Senescence TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
Fc epsilon receptor (FCERI) signaling TAS Reactome
FCERI mediated MAPK activation TAS Reactome
Immune System TAS Reactome
Innate Immune System TAS Reactome
Interleukin-17 signaling TAS Reactome
JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 TAS Reactome
MAP kinase activation TAS Reactome
MAPK targets/ Nuclear events mediated by MAP kinases TAS Reactome
MyD88 cascade initiated on plasma membrane TAS Reactome
MyD88 dependent cascade initiated on endosome TAS Reactome
MyD88-independent TLR4 cascade TAS Reactome
MyD88:MAL(TIRAP) cascade initiated on plasma membrane TAS Reactome
Oxidative Stress Induced Senescence TAS Reactome
Signaling by Interleukins TAS Reactome
Toll Like Receptor 10 (TLR10) Cascade TAS Reactome
Toll Like Receptor 2 (TLR2) Cascade TAS Reactome
Toll Like Receptor 3 (TLR3) Cascade TAS Reactome
Toll Like Receptor 4 (TLR4) Cascade TAS Reactome
Toll Like Receptor 5 (TLR5) Cascade TAS Reactome
Toll Like Receptor 7/8 (TLR7/8) Cascade TAS Reactome
Toll Like Receptor 9 (TLR9) Cascade TAS Reactome
Toll Like Receptor TLR1:TLR2 Cascade TAS Reactome
Toll Like Receptor TLR6:TLR2 Cascade TAS Reactome
Toll-like Receptor Cascades TAS Reactome
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation TAS Reactome
TRIF (TICAM1)-mediated TLR4 signaling TAS Reactome





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