Gene description for MAPK10
Gene name mitogen-activated protein kinase 10
Gene symbol MAPK10
Other names/aliases JNK3
JNK3A
PRKM10
SAPK1b
p493F12
p54bSAPK
Species Homo sapiens
 Database cross references - MAPK10
ExoCarta ExoCarta_5602
Vesiclepedia VP_5602
Entrez Gene 5602
HGNC 6872
MIM 602897
UniProt P53779  
 MAPK10 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for MAPK10
Molecular Function
    protein binding GO:0005515 IPI
    MAP kinase kinase activity GO:0004708 TAS
    JUN kinase activity GO:0004705 ISS
    ATP binding GO:0005524 IEA
Biological Process
    innate immune response GO:0045087 TAS
    toll-like receptor 2 signaling pathway GO:0034134 TAS
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    toll-like receptor TLR1:TLR2 signaling pathway GO:0038123 TAS
    JUN phosphorylation GO:0007258 TAS
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    regulation of sequence-specific DNA binding transcription factor activity GO:0051090 TAS
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    stress-activated MAPK cascade GO:0051403 TAS
    response to light stimulus GO:0009416 ISS
    toll-like receptor 5 signaling pathway GO:0034146 TAS
    toll-like receptor signaling pathway GO:0002224 TAS
    JNK cascade GO:0007254 TAS
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
    signal transduction GO:0007165 TAS
    toll-like receptor TLR6:TLR2 signaling pathway GO:0038124 TAS
    regulation of circadian rhythm GO:0042752 ISS
    activation of MAPK activity GO:0000187 TAS
    toll-like receptor 9 signaling pathway GO:0034162 TAS
    rhythmic process GO:0048511 IEA
    protein phosphorylation GO:0006468 ISS
    toll-like receptor 10 signaling pathway GO:0034166 TAS
Subcellular Localization
    plasma membrane GO:0005886 ISS
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 TAS
    mitochondrion GO:0005739 ISS
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified MAPK10 in exosomes
1
Experiment ID 237
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MAPK10
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAPK8IP3  
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
2 UNC119 9094
Two-hybrid Homo sapiens
3 GTF3C1  
Two-hybrid Homo sapiens
4 ARRB2 409
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
5 DUSP1  
Affinity Capture-Western Homo sapiens
6 SH3BP5 9467
Reconstituted Complex Homo sapiens
7 DUSP10 11221
Affinity Capture-Western Homo sapiens
8 ARRB1 408
Affinity Capture-Western Homo sapiens
9 TP53 7157
Invivo Homo sapiens
10 STMN2  
Invitro Homo sapiens
11 DUSP16  
Affinity Capture-Western Homo sapiens
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