Gene description for PABPC3
Gene name poly(A) binding protein, cytoplasmic 3
Gene symbol PABPC3
Other names/aliases PABP3
PABPL3
tPABP
Species Homo sapiens
 Database cross references - PABPC3
ExoCarta ExoCarta_5042
Vesiclepedia VP_5042
Entrez Gene 5042
HGNC 8556
MIM 604680
UniProt Q9H361  
 PABPC3 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Neuroblastoma cells 25944692    
 Gene ontology annotations for PABPC3
Molecular Function
    mRNA 3'-UTR binding GO:0003730 IBA
    protein binding GO:0005515 IPI
    poly(A) binding GO:0008143 IBA
    poly(A) binding GO:0008143 IDA
    poly(U) RNA binding GO:0008266 IBA
Biological Process
    mRNA metabolic process GO:0016071 NAS
Subcellular Localization
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 NAS
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytoplasmic stress granule GO:0010494 IBA
    extracellular exosome GO:0070062 HDA
    ribonucleoprotein complex GO:1990904 IBA
 Experiment description of studies that identified PABPC3 in exosomes
1
Experiment ID 234
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 236
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
4
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for PABPC3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APP 351
Reconstituted Complex Homo sapiens
2 FAM46D  
Two-hybrid Homo sapiens
3 CNBP 7555
Co-fractionation Homo sapiens
4 ANLN 54443
Affinity Capture-MS Homo sapiens
5 NFATC3 4775
Affinity Capture-MS Homo sapiens
6 ARRB2 409
Affinity Capture-MS Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 PAPOLA 10914
Co-fractionation Homo sapiens
9 TP53 7157
Affinity Capture-MS Homo sapiens
10 HIST1H3A 8350
Cross-Linking-MS (XL-MS) Homo sapiens
11 SOD1 6647
Co-fractionation Homo sapiens
12 EEF1A1 1915
Co-fractionation Homo sapiens
13 KIF14 9928
Affinity Capture-MS Homo sapiens
14 PAN3 255967
Co-fractionation Homo sapiens
15 RC3H2  
Affinity Capture-MS Homo sapiens
16 WWOX 51741
Affinity Capture-MS Homo sapiens
17 BRIX1 55299
Co-fractionation Homo sapiens
18 ZNF746  
Affinity Capture-MS Homo sapiens
19 KIF20A 10112
Affinity Capture-MS Homo sapiens
20 MKRN3  
Affinity Capture-MS Homo sapiens
21 CRY1  
Affinity Capture-MS Homo sapiens
22 ECT2 1894
Affinity Capture-MS Homo sapiens
23 PARK2  
Affinity Capture-MS Homo sapiens
24 EIF4E 1977
Co-fractionation Homo sapiens
25 PEBP1 5037
Co-fractionation Homo sapiens
26 GALK2 2585
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 GAN 8139
Affinity Capture-MS Homo sapiens
28 FAM109A  
Affinity Capture-MS Homo sapiens
29 GTF3C4 9329
Affinity Capture-MS Homo sapiens
30 TRIM54  
Two-hybrid Homo sapiens
31 TFCP2 7024
Affinity Capture-MS Homo sapiens
32 AURKB 9212
Affinity Capture-MS Homo sapiens
33 CHMP4C 92421
Affinity Capture-MS Homo sapiens
34 RUVBL1 8607
Affinity Capture-MS Homo sapiens
35 CHMP4B 128866
Affinity Capture-MS Homo sapiens
36 CSTF3 1479
Co-fractionation Homo sapiens
37 RPS13 6207
Cross-Linking-MS (XL-MS) Homo sapiens
38 KIF23 9493
Affinity Capture-MS Homo sapiens
39 MCM2 4171
Affinity Capture-MS Homo sapiens
40 TMOD2 29767
Co-fractionation Homo sapiens
41 PABPC1 26986
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
42 NHP2L1 4809
Co-fractionation Homo sapiens
43 EEF1A2 1917
Co-fractionation Homo sapiens
44 PRC1 9055
Affinity Capture-MS Homo sapiens
45 PABPC1L 80336
Cross-Linking-MS (XL-MS) Homo sapiens
46 C9orf72  
Affinity Capture-MS Homo sapiens
47 CEMIP 57214
Affinity Capture-MS Homo sapiens
48 KRTAP13-3  
Affinity Capture-MS Homo sapiens
49 RC3H1 149041
Affinity Capture-MS Homo sapiens
50 YAP1 10413
Affinity Capture-MS Homo sapiens
51 ZC3H3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 NFX1  
Affinity Capture-MS Homo sapiens
53 MGMT 4255
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PABPC3 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here