Gene description for MMP3
Gene name matrix metallopeptidase 3
Gene symbol MMP3
Other names/aliases CHDS6
MMP-3
SL-1
STMY
STMY1
STR1
Species Homo sapiens
 Database cross references - MMP3
ExoCarta ExoCarta_4314
Vesiclepedia VP_4314
Entrez Gene 4314
HGNC 7173
MIM 185250
UniProt P08254  
 MMP3 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for MMP3
Molecular Function
    endopeptidase activity GO:0004175 IDA
    metalloendopeptidase activity GO:0004222 IBA
    metalloendopeptidase activity GO:0004222 TAS
    serine-type endopeptidase activity GO:0004252 TAS
    protein binding GO:0005515 IPI
    peptidase activity GO:0008233 IDA
    metallopeptidase activity GO:0008237 IDA
    zinc ion binding GO:0008270 IEA
Biological Process
    proteolysis GO:0006508 IDA
    extracellular matrix disassembly GO:0022617 TAS
    protein catabolic process GO:0030163 IEA
    extracellular matrix organization GO:0030198 IBA
    regulation of cell migration GO:0030334 IEA
    collagen catabolic process GO:0030574 IBA
    collagen catabolic process GO:0030574 TAS
    positive regulation of protein-containing complex assembly GO:0031334 IDA
    cellular response to reactive oxygen species GO:0034614 ISS
    innate immune response GO:0045087 IEA
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 IEA
    cellular response to lipopolysaccharide GO:0071222 TAS
    cellular response to amino acid stimulus GO:0071230 IEA
    cellular response to UV-A GO:0071492 IDA
    cellular response to nitric oxide GO:0071732 TAS
    regulation of neuroinflammatory response GO:0150077 TAS
    response to amyloid-beta GO:1904645 TAS
    negative regulation of reactive oxygen species metabolic process GO:2000378 IDA
Subcellular Localization
    extracellular region GO:0005576 TAS
    nucleus GO:0005634 IEA
    mitochondrion GO:0005739 IEA
    cytosol GO:0005829 IEA
    extracellular matrix GO:0031012 IEA
 Experiment description of studies that identified MMP3 in exosomes
1
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Antibody array
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for MMP3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BMP4 652
Affinity Capture-MS Homo sapiens
2 MEOX2  
Two-hybrid Homo sapiens
3 NMU  
Affinity Capture-MS Homo sapiens
4 LTBP1 4052
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 SPOCK3  
Affinity Capture-Western Homo sapiens
6 CLN5  
Affinity Capture-MS Homo sapiens
7 APOE 348
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 KIAA0319L 79932
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 BCAN  
Biochemical Activity Homo sapiens
10 HBD 3045
Affinity Capture-MS Homo sapiens
11 TIMP1 7076
Co-crystal Structure Homo sapiens
12 PTPRS 5802
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 TIMP3 7078
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 NUCB1 4924
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 CLU 1191
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 MMP10 4319
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 TIGD7  
Affinity Capture-MS Homo sapiens
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