Gene description for PROS1
Gene name protein S (alpha)
Gene symbol PROS1
Other names/aliases PROS
PS21
PS22
PS23
PS24
PS25
PSA
THPH5
THPH6
Species Homo sapiens
 Database cross references - PROS1
ExoCarta ExoCarta_5627
Entrez Gene 5627
HGNC 9456
MIM 176880
UniProt P07225  
 PROS1 identified in exosomes derived from the following tissue/cell type
Plasma 19028452    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for PROS1
Molecular Function
    calcium ion binding GO:0005509 IEA
    endopeptidase inhibitor activity GO:0004866 TAS
Biological Process
    proteolysis GO:0006508 TAS
    platelet degranulation GO:0002576 TAS
    fibrinolysis GO:0042730 IEA
    leukocyte migration GO:0050900 TAS
    regulation of complement activation GO:0030449 TAS
    peptidyl-glutamic acid carboxylation GO:0017187 TAS
    post-translational protein modification GO:0043687 TAS
    negative regulation of endopeptidase activity GO:0010951 TAS
    innate immune response GO:0045087 TAS
    blood coagulation GO:0007596 TAS
    cellular protein metabolic process GO:0044267 TAS
    platelet activation GO:0030168 TAS
Subcellular Localization
    platelet alpha granule lumen GO:0031093 TAS
    plasma membrane GO:0005886 TAS
    endoplasmic reticulum membrane GO:0005789 TAS
    extracellular region GO:0005576 TAS
    blood microparticle GO:0072562 IDA
    extracellular exosome GO:0070062 IDA
    extracellular space GO:0005615 IDA
    Golgi membrane GO:0000139 TAS
    Golgi lumen GO:0005796 TAS
 Experiment description of studies that identified PROS1 in exosomes
1
Experiment ID 44
ISEV standards
✔
IEM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19028452    
Organism Homo sapiens
Experiment description Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein.
Authors Looze C, Yui D, Leung L, Ingham M, Kaler M, Yao X, Wu WW, Shen RF, Daniels MP, Levine SJ
Journal name BBRC
Publication year 2009
Sample Plasma
Sample name Plasma
Isolation/purification methods Filtration
Size exclusion
Sucrose density gradient
Flotation density 1.15-1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
2
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PROS1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PROC  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
2 C4BPA 722
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
3 TYRO3  
Invivo Homo sapiens
4 F5 2153
Invitro Homo sapiens
Invitro Homo sapiens
5 F8 2157
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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