Gene description for PCSK6
Gene name proprotein convertase subtilisin/kexin type 6
Gene symbol PCSK6
Other names/aliases PACE4
SPC4
Species Homo sapiens
 Database cross references - PCSK6
ExoCarta ExoCarta_5046
Entrez Gene 5046
HGNC 8569
MIM 167405
UniProt P29122  
 PCSK6 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
 Gene ontology annotations for PCSK6
Molecular Function
    endopeptidase activity GO:0004175 IDA
    nerve growth factor binding GO:0048406 IDA
    serine-type endopeptidase activity GO:0004252 IDA
    heparin binding GO:0008201 IDA
Biological Process
    peptide hormone processing GO:0016486 IDA
    zygotic determination of anterior/posterior axis, embryo GO:0007354 IEA
    nerve growth factor processing GO:0032455 TAS
    secretion by cell GO:0032940 IDA
    nerve growth factor production GO:0032902 IDA
    glycoprotein metabolic process GO:0009100 IDA
    regulation of BMP signaling pathway GO:0030510 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    protein processing GO:0016485 IDA
    determination of left/right symmetry GO:0007368 IEA
Subcellular Localization
    extracellular matrix GO:0031012 IDA
    endoplasmic reticulum GO:0005783 IEA
    extracellular space GO:0005615 IDA
    membrane GO:0016020 IEA
    cell surface GO:0009986 IDA
    Golgi lumen GO:0005796 TAS
 Experiment description of studies that identified PCSK6 in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PCSK6
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which PCSK6 is involved
PathwayEvidenceSource
NGF processing TAS Reactome
Signaling by NODAL TAS Reactome





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