Gene description for PCSK6
Gene name proprotein convertase subtilisin/kexin type 6
Gene symbol PCSK6
Other names/aliases PACE4
SPC4
Species Homo sapiens
 Database cross references - PCSK6
ExoCarta ExoCarta_5046
Vesiclepedia VP_5046
Entrez Gene 5046
HGNC 8569
MIM 167405
UniProt P29122  
 PCSK6 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
 Gene ontology annotations for PCSK6
Molecular Function
    endopeptidase activity GO:0004175 IDA
    endopeptidase activity GO:0004175 TAS
    serine-type endopeptidase activity GO:0004252 IBA
    serine-type endopeptidase activity GO:0004252 IDA
    protein binding GO:0005515 IPI
    heparin binding GO:0008201 IDA
    nerve growth factor binding GO:0048406 IDA
Biological Process
    zygotic determination of anterior/posterior axis, embryo GO:0007354 IEA
    determination of left/right symmetry GO:0007368 IEA
    glycoprotein metabolic process GO:0009100 IDA
    protein processing GO:0016485 IDA
    peptide hormone processing GO:0016486 IBA
    peptide hormone processing GO:0016486 IDA
    regulation of BMP signaling pathway GO:0030510 TAS
    nerve growth factor production GO:0032902 IDA
    secretion by cell GO:0032940 IDA
    plasma lipoprotein particle remodeling GO:0034369 TAS
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    endoplasmic reticulum GO:0005783 IEA
    Golgi lumen GO:0005796 TAS
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IBA
    cell surface GO:0009986 IDA
    membrane GO:0016020 IBA
    collagen-containing extracellular matrix GO:0062023 IBA
    collagen-containing extracellular matrix GO:0062023 IDA
 Experiment description of studies that identified PCSK6 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PCSK6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LY86  
Affinity Capture-MS Homo sapiens
2 LY6H  
Affinity Capture-MS Homo sapiens
3 GPIHBP1  
Affinity Capture-MS Homo sapiens
4 ELSPBP1  
Affinity Capture-MS Homo sapiens
5 EPDR1 54749
Affinity Capture-MS Homo sapiens
6 KLK15  
Affinity Capture-MS Homo sapiens
7 C1QTNF9B  
Affinity Capture-MS Homo sapiens
8 TMEM87A 25963
Affinity Capture-MS Homo sapiens
9 ZNF441  
Two-hybrid Homo sapiens
10 LRRC59 55379
Proximity Label-MS Homo sapiens
11 EPHA7 2045
Affinity Capture-MS Homo sapiens
12 TMEM25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 FSTL4  
Affinity Capture-MS Homo sapiens
14 GGH 8836
Affinity Capture-MS Homo sapiens
15 TRIM66  
Affinity Capture-MS Homo sapiens
16 PLD6  
Affinity Capture-MS Homo sapiens
17 TRGV3  
Affinity Capture-MS Homo sapiens
18 PRG2  
Affinity Capture-MS Homo sapiens
19 MFAP4 4239
Affinity Capture-MS Homo sapiens
20 CUL3 8452
Affinity Capture-MS Homo sapiens
21 TSHR 7253
Affinity Capture-MS Homo sapiens
22 CBLN4  
Affinity Capture-MS Homo sapiens
23 LYZL1  
Affinity Capture-MS Homo sapiens
24 NCR3  
Affinity Capture-MS Homo sapiens
25 IL5RA  
Affinity Capture-MS Homo sapiens
26 KLK2  
Affinity Capture-MS Homo sapiens
27 KLK1 3816
Affinity Capture-MS Homo sapiens
28 MSTN  
Affinity Capture-MS Homo sapiens
29 C1QB 713
Affinity Capture-MS Homo sapiens
30 SIRPD  
Affinity Capture-MS Homo sapiens
31 IDS  
Affinity Capture-MS Homo sapiens
32 SDF2L1 23753
Affinity Capture-MS Homo sapiens
33 FIBIN  
Affinity Capture-MS Homo sapiens
34 EFNA2  
Affinity Capture-MS Homo sapiens
35 CRISP2  
Affinity Capture-MS Homo sapiens
36 BTLA  
Affinity Capture-MS Homo sapiens
37 ALPP 250
Affinity Capture-MS Homo sapiens
38 DNAJB9 4189
Affinity Capture-MS Homo sapiens
39 NAGA 4668
Affinity Capture-MS Homo sapiens
40 CACNA1A  
Two-hybrid Homo sapiens
41 C4BPB 725
Affinity Capture-MS Homo sapiens
42 EDN3  
Affinity Capture-MS Homo sapiens
43 CFC1  
Affinity Capture-MS Homo sapiens
44 BRICD5  
Affinity Capture-MS Homo sapiens
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