Gene description for RBSN
Gene name rabenosyn, RAB effector
Gene symbol RBSN
Other names/aliases Rabenosyn-5
ZFYVE20
Species Homo sapiens
 Database cross references - RBSN
ExoCarta ExoCarta_64145
Vesiclepedia VP_64145
Entrez Gene 64145
HGNC 20759
MIM 609511
UniProt Q9H1K0  
 RBSN identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for RBSN
Molecular Function
    zinc ion binding GO:0008270 NAS
    protein binding GO:0005515 IPI
    Rab GTPase binding GO:0017137 IBA
Biological Process
    endocytosis GO:0006897 IBA
    late endosome to vacuole transport GO:0045324 IBA
    endosomal transport GO:0016197 NAS
    protein transport GO:0015031 IEA
    endosomal vesicle fusion GO:0034058 IBA
    Golgi to vacuole transport GO:0006896 IBA
    blood coagulation GO:0007596 TAS
    Golgi to endosome transport GO:0006895 IBA
    vacuole inheritance GO:0000011 IBA
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    endosome membrane GO:0010008 TAS
    intracellular membrane-bounded organelle GO:0043231 IDA
    endosome GO:0005768 NAS
    early endosome membrane GO:0031901 IEA
    cytoplasmic side of endosome membrane GO:0010009 IBA
    plasma membrane GO:0005886 IEA
 Experiment description of studies that identified RBSN in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for RBSN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAB5A 5868
Invitro Homo sapiens
2 VPS45 11311
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which RBSN is involved
PathwayEvidenceSource
Factors involved in megakaryocyte development and platelet production TAS Reactome





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