Gene description for FBP1
Gene name fructose-1,6-bisphosphatase 1
Gene symbol FBP1
Other names/aliases FBP
Species Homo sapiens
 Database cross references - FBP1
ExoCarta ExoCarta_2203
Vesiclepedia VP_2203
Entrez Gene 2203
HGNC 3606
MIM 611570
UniProt P09467  
 FBP1 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 25890246    
Colorectal cancer cells 34887515    
Mesenchymal stem cells 36408942    
Urine 19056867    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for FBP1
Molecular Function
    protein binding GO:0005515 IPI
    AMP binding GO:0016208 IDA
    fructose 1,6-bisphosphate 1-phosphatase activity GO:0042132 IBA
    fructose 1,6-bisphosphate 1-phosphatase activity GO:0042132 IDA
    fructose 1,6-bisphosphate 1-phosphatase activity GO:0042132 IMP
    identical protein binding GO:0042802 IPI
    metal ion binding GO:0046872 IMP
    monosaccharide binding GO:0048029 ISS
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IMP
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IMP
    fructose metabolic process GO:0006000 IBA
    fructose 6-phosphate metabolic process GO:0006002 IBA
    fructose 6-phosphate metabolic process GO:0006002 IDA
    gluconeogenesis GO:0006094 IBA
    gluconeogenesis GO:0006094 IEA
    gluconeogenesis GO:0006094 IMP
    gluconeogenesis GO:0006094 ISS
    regulation of gluconeogenesis GO:0006111 IMP
    dephosphorylation GO:0016311 IDA
    dephosphorylation GO:0016311 IMP
    negative regulation of cell growth GO:0030308 IDA
    fructose 1,6-bisphosphate metabolic process GO:0030388 IBA
    response to nutrient levels GO:0031667 IEA
    cellular response to insulin stimulus GO:0032869 IEA
    negative regulation of glycolytic process GO:0045820 IDA
    negative regulation of glycolytic process GO:0045820 IMP
    negative regulation of Ras protein signal transduction GO:0046580 IDA
    cellular response to magnesium ion GO:0071286 IDA
    cellular response to cAMP GO:0071320 IEA
    cellular response to xenobiotic stimulus GO:0071466 IDA
    cellular hyperosmotic salinity response GO:0071475 IEA
    cellular hypotonic salinity response GO:0071477 IEA
    cellular response to raffinose GO:0097403 IEA
    cellular response to phorbol 13-acetate 12-myristate GO:1904628 IEA
Subcellular Localization
    nucleus GO:0005634 IMP
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISS
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified FBP1 in sEVs
1
Experiment ID 282
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
3
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
5
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for FBP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RELA 5970
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
2 KLRC2  
Two-hybrid Homo sapiens
3 CCDC68  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 DIDO1  
Affinity Capture-MS Homo sapiens
5 CSGALNACT1 55790
Affinity Capture-MS Homo sapiens
6 POT1  
Two-hybrid Homo sapiens
7 ST6GALNAC5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 HSPA8 3312
Two-hybrid Homo sapiens
9 RNF183  
Two-hybrid Homo sapiens
10 HDAC6 10013
Two-hybrid Homo sapiens
11 DNMT1 1786
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
12 ATP5A1 498
Co-fractionation Homo sapiens
13 DYNC1I1  
Two-hybrid Homo sapiens
14 CAPZA2 830
Affinity Capture-MS Homo sapiens
15 LNX1  
Two-hybrid Homo sapiens
16 ILDR1 286676
Affinity Capture-MS Homo sapiens
17 PARK2  
Biochemical Activity Homo sapiens
18 PTK2 5747
Two-hybrid Homo sapiens
19 ASL 435
Two-hybrid Homo sapiens
20 CROT 54677
Affinity Capture-MS Homo sapiens
21 GIMAP2  
Affinity Capture-MS Homo sapiens
22 ATP5J2 9551
Two-hybrid Homo sapiens
23 PIM2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
24 TERF1 7013
Two-hybrid Homo sapiens
25 TRIM28 10155
Affinity Capture-MS Homo sapiens
26 BCL2L1 598
Two-hybrid Homo sapiens
27 FXR2 9513
Two-hybrid Homo sapiens
28 FBP1 2203
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
29 USP7 7874
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
30 TIGD7  
Affinity Capture-MS Homo sapiens
31 ASCC2 84164
Two-hybrid Homo sapiens
32 SAMD7  
Affinity Capture-MS Homo sapiens
33 IQGAP1 8826
Affinity Capture-MS Homo sapiens
34 CSNK1E 1454
Two-hybrid Homo sapiens
35 ACTR1A 10121
Affinity Capture-MS Homo sapiens
36 FBP2 8789
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
37 MGC39584  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 SLAMF8  
Affinity Capture-MS Homo sapiens
39 PCNXL4 64430
Two-hybrid Homo sapiens
40 BIN1 274
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Protein-peptide Homo sapiens
41 MED24 9862
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which FBP1 is involved
PathwayEvidenceSource
Gluconeogenesis TAS Reactome
Glucose metabolism TAS Reactome
Metabolism TAS Reactome
Metabolism of carbohydrates TAS Reactome





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