Gene description for HES1
Gene name hes family bHLH transcription factor 1
Gene symbol HES1
Other names/aliases HES-1
HHL
HRY
bHLHb39
Species Homo sapiens
 Database cross references - HES1
ExoCarta ExoCarta_3280
Vesiclepedia VP_3280
Entrez Gene 3280
HGNC 5192
MIM 139605
UniProt Q14469  
 HES1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for HES1
Molecular Function
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    transcription corepressor binding GO:0001222 IEA
    DNA-binding transcription repressor activity, RNA polymerase II-specific GO:0001227 ISS
    DNA binding GO:0003677 TAS
    chromatin binding GO:0003682 IEA
    protein binding GO:0005515 IPI
    JUN kinase binding GO:0008432 IEA
    protein homodimerization activity GO:0042803 ISS
    histone deacetylase binding GO:0042826 IPI
    HLH domain binding GO:0043398 IEA
    sequence-specific DNA binding GO:0043565 ISS
    protein-containing complex binding GO:0044877 IEA
    protein-folding chaperone binding GO:0051087 IEA
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 ISS
    E-box binding GO:0070888 IBA
    E-box binding GO:0070888 ISS
    N-box binding GO:0071820 IBA
    N-box binding GO:0071820 ISS
    sequence-specific double-stranded DNA binding GO:1990837 IDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    cell fate determination GO:0001709 IEA
    liver development GO:0001889 IEA
    embryonic heart tube morphogenesis GO:0003143 ISS
    outflow tract morphogenesis GO:0003151 ISS
    regulation of secondary heart field cardioblast proliferation GO:0003266 IEA
    ventricular septum development GO:0003281 ISS
    pancreatic A cell differentiation GO:0003310 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    cell adhesion GO:0007155 IEA
    Notch signaling pathway GO:0007219 IBA
    Notch signaling pathway GO:0007219 IDA
    Notch signaling pathway GO:0007219 IMP
    smoothened signaling pathway GO:0007224 IEA
    nervous system development GO:0007399 TAS
    positive regulation of cell population proliferation GO:0008284 ISS
    anterior/posterior pattern specification GO:0009952 IBA
    positive regulation of gene expression GO:0010628 IEA
    negative regulation of gene expression GO:0010629 IEA
    negative regulation of neuron projection development GO:0010977 IEA
    response to organic cyclic compound GO:0014070 IEA
    cell migration GO:0016477 IEA
    telencephalon development GO:0021537 IEA
    midbrain-hindbrain boundary morphogenesis GO:0021555 IEA
    oculomotor nerve development GO:0021557 IEA
    trochlear nerve development GO:0021558 IEA
    hindbrain morphogenesis GO:0021575 IEA
    forebrain radial glial cell differentiation GO:0021861 ISS
    Cajal-Retzius cell differentiation GO:0021870 IEA
    adenohypophysis development GO:0021984 IEA
    lung development GO:0030324 IEA
    BMP signaling pathway GO:0030509 IEA
    positive regulation of BMP signaling pathway GO:0030513 IEA
    midbrain development GO:0030901 IEA
    somatic stem cell population maintenance GO:0035019 IEA
    amacrine cell differentiation GO:0035881 IEA
    ascending aorta morphogenesis GO:0035910 ISS
    T cell proliferation GO:0042098 IEA
    positive regulation of T cell proliferation GO:0042102 IEA
    inner ear auditory receptor cell differentiation GO:0042491 IEA
    positive regulation of tyrosine phosphorylation of STAT protein GO:0042531 ISS
    regulation of protein-containing complex assembly GO:0043254 IEA
    response to alkaloid GO:0043279 IEA
    positive regulation of DNA binding GO:0043388 ISS
    negative regulation of DNA-binding transcription factor activity GO:0043433 ISS
    regulation of fat cell differentiation GO:0045598 IEA
    negative regulation of inner ear auditory receptor cell differentiation GO:0045608 IEA
    negative regulation of neuron differentiation GO:0045665 IBA
    positive regulation of Notch signaling pathway GO:0045747 IEA
    negative regulation of DNA-templated transcription GO:0045892 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    positive regulation of mitotic cell cycle, embryonic GO:0045977 ISS
    lateral inhibition GO:0046331 IEA
    regulation of receptor signaling pathway via JAK-STAT GO:0046425 ISS
    positive regulation of receptor signaling pathway via JAK-STAT GO:0046427 ISS
    cell maturation GO:0048469 IEA
    thymus development GO:0048538 ISS
    cell morphogenesis involved in neuron differentiation GO:0048667 IEA
    positive regulation of astrocyte differentiation GO:0048711 ISS
    negative regulation of oligodendrocyte differentiation GO:0048715 ISS
    artery morphogenesis GO:0048844 ISS
    regulation of epithelial cell proliferation GO:0050678 IEA
    regulation of neurogenesis GO:0050767 IBA
    inner ear receptor cell stereocilium organization GO:0060122 IEA
    regulation of timing of neuron differentiation GO:0060164 IEA
    negative regulation of glial cell proliferation GO:0060253 ISS
    ventricular septum morphogenesis GO:0060412 ISS
    ureteric bud morphogenesis GO:0060675 IEA
    labyrinthine layer blood vessel development GO:0060716 IEA
    common bile duct development GO:0061009 IEA
    stomach neuroendocrine cell differentiation GO:0061102 IEA
    negative regulation of stomach neuroendocrine cell differentiation GO:0061106 IEA
    cardiac neural crest cell development involved in outflow tract morphogenesis GO:0061309 IEA
    pharyngeal arch artery morphogenesis GO:0061626 ISS
    protein-containing complex assembly GO:0065003 ISS
    cellular response to interleukin-1 GO:0071347 IEA
    cellular response to tumor necrosis factor GO:0071356 IEA
    cellular response to fatty acid GO:0071398 IEA
    glomerulus vasculature development GO:0072012 IEA
    comma-shaped body morphogenesis GO:0072049 IEA
    S-shaped body morphogenesis GO:0072050 IEA
    renal interstitial fibroblast development GO:0072141 IEA
    metanephric nephron tubule morphogenesis GO:0072282 IEA
    cochlea development GO:0090102 IEA
    establishment of epithelial cell polarity GO:0090162 IEA
    negative regulation of calcium ion import GO:0090281 IEA
    response to thyroid hormone GO:0097066 IEA
    vascular associated smooth muscle cell development GO:0097084 ISS
    neuronal stem cell population maintenance GO:0097150 IEP
    negative regulation of amacrine cell differentiation GO:1902870 IEA
    negative regulation of cell fate determination GO:1905934 IEA
    cellular response to nerve growth factor stimulus GO:1990090 IEA
    negative regulation of pancreatic A cell differentiation GO:2000227 IEA
    negative regulation of stem cell differentiation GO:2000737 IMP
    negative regulation of pro-B cell differentiation GO:2000974 IMP
    negative regulation of forebrain neuron differentiation GO:2000978 ISS
Subcellular Localization
    chromatin GO:0000785 IEA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IEA
    nuclear matrix GO:0016363 IEA
    protein-containing complex GO:0032991 IEA
 Experiment description of studies that identified HES1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for HES1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FBXL14  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 FHL1 2273
Two-hybrid Homo sapiens
3 CUL1 8454
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
4 SPTAN1 6709
Affinity Capture-MS Homo sapiens
5 PARP1 142
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
6 NR4A1  
Two-hybrid Homo sapiens
7 RBPJ 3516
Co-crystal Structure Homo sapiens
8 FANCC 2176
Affinity Capture-Western Homo sapiens
9 TLE2  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
10 YWHAB 7529
Two-hybrid Homo sapiens
11 LTBR 4055
Two-hybrid Homo sapiens
12 RB1 5925
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
13 HDAC6 10013
Two-hybrid Homo sapiens
14 FANCL 55120
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
15 ASGR2  
Two-hybrid Homo sapiens
16 TLE1 7088
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
17 SKP1 6500
Affinity Capture-Western Homo sapiens
18 UBC 7316
Affinity Capture-Western Homo sapiens
19 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
20 HDAC1 3065
Affinity Capture-Western Homo sapiens
21 PTK2 5747
Two-hybrid Homo sapiens
22 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
23 SIRT1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
24 APCS 325
Two-hybrid Homo sapiens
25 HEY2  
Reconstituted Complex Homo sapiens
26 FANCE  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
27 ZNF70  
Co-localization Homo sapiens
28 FANCA  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
29 ZFP64  
Co-localization Homo sapiens
30 GAPDH 2597
Two-hybrid Homo sapiens
31 FANCG 2189
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
32 FANCF  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
33 RUNX2  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
34 HES1 3280
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
35 HES6  
Affinity Capture-MS Homo sapiens
36 HEY1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 HSPA4 3308
Affinity Capture-MS Homo sapiens
38 NUDT3 11165
Two-hybrid Homo sapiens
39 HMGCL 3155
Two-hybrid Homo sapiens
40 CSNK1E 1454
Two-hybrid Homo sapiens
41 APH1A 51107
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which HES1 is involved
PathwayEvidenceSource
Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants TAS Reactome
Constitutive Signaling by NOTCH1 PEST Domain Mutants TAS Reactome
Developmental Biology IEA Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
Gene expression (Transcription) IEA Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway IEA Reactome
Generic Transcription Pathway TAS Reactome
NOTCH1 Intracellular Domain Regulates Transcription IEA Reactome
NOTCH2 intracellular domain regulates transcription IEA Reactome
NOTCH3 Intracellular Domain Regulates Transcription IEA Reactome
NOTCH4 Intracellular Domain Regulates Transcription TAS Reactome
Regulation of beta-cell development IEA Reactome
Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells IEA Reactome
RNA Polymerase II Transcription IEA Reactome
RNA Polymerase II Transcription TAS Reactome
RUNX2 regulates bone development IEA Reactome
RUNX2 regulates osteoblast differentiation IEA Reactome
RUNX3 regulates NOTCH signaling IEA Reactome
RUNX3 regulates NOTCH signaling TAS Reactome
Signal Transduction IEA Reactome
Signal Transduction TAS Reactome
Signaling by NOTCH IEA Reactome
Signaling by NOTCH TAS Reactome
Signaling by NOTCH1 IEA Reactome
Signaling by NOTCH1 HD+PEST Domain Mutants in Cancer TAS Reactome
Signaling by NOTCH1 in Cancer TAS Reactome
Signaling by NOTCH1 PEST Domain Mutants in Cancer TAS Reactome
Signaling by NOTCH2 IEA Reactome
Signaling by NOTCH3 IEA Reactome
Signaling by NOTCH4 TAS Reactome
Transcriptional regulation by RUNX2 IEA Reactome
Transcriptional regulation by RUNX3 IEA Reactome
Transcriptional regulation by RUNX3 TAS Reactome





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