Gene description for EXOC2
Gene name exocyst complex component 2
Gene symbol EXOC2
Other names/aliases SEC5
SEC5L1
Sec5p
Species Homo sapiens
 Database cross references - EXOC2
ExoCarta ExoCarta_55770
Entrez Gene 55770
HGNC 24968
MIM 615329
UniProt Q96KP1  
 EXOC2 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for EXOC2
Molecular Function
    Ral GTPase binding GO:0017160 IPI
    protein N-terminus binding GO:0047485 IEA
    protein kinase binding GO:0019901 IPI
    protein binding GO:0005515 IPI
Biological Process
    organelle organization GO:0006996 TAS
    membrane organization GO:0061024 TAS
    regulation of entry of bacterium into host cell GO:2000535 IMP
    exocytosis GO:0006887 IEA
    protein transport GO:0015031 IEA
    Golgi to plasma membrane transport GO:0006893 IEA
    cellular protein metabolic process GO:0044267 TAS
Subcellular Localization
    intracellular membrane-bounded organelle GO:0043231 IDA
    membrane GO:0016020 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 TAS
    exocyst GO:0000145 IDA
 Experiment description of studies that identified EXOC2 in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EXOC2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RALB 5899
Two-hybrid Homo sapiens
2 EXOC8 149371
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
3 DELGEF  
Invitro Homo sapiens
Two-hybrid Homo sapiens
4 RALA 5898
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which EXOC2 is involved
PathwayEvidenceSource
Insulin processing TAS Reactome
Translocation of GLUT4 to the plasma membrane TAS Reactome
VxPx cargo-targeting to cilium TAS Reactome





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