Gene description for SPRR3
Gene name small proline-rich protein 3
Gene symbol SPRR3
Other names/aliases -
Species Homo sapiens
 Database cross references - SPRR3
ExoCarta ExoCarta_6707
Vesiclepedia VP_6707
Entrez Gene 6707
HGNC 11268
MIM 182271
UniProt Q9UBC9  
 SPRR3 identified in sEVs derived from the following tissue/cell type
Bladder cancer cells 20224111    
Saliva 19199708    
Urine 19056867    
 Gene ontology annotations for SPRR3
Molecular Function
    structural molecule activity GO:0005198 TAS
    protein binding GO:0005515 IPI
Biological Process
    epidermis development GO:0008544 NAS
    keratinocyte differentiation GO:0030216 NAS
    keratinization GO:0031424 IEA
    wound healing GO:0042060 TAS
Subcellular Localization
    cornified envelope GO:0001533 TAS
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 TAS
    perinuclear region of cytoplasm GO:0048471 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified SPRR3 in sEVs
1
Experiment ID 76
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for SPRR3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LY86  
Affinity Capture-MS Homo sapiens
2 FNBP4  
Affinity Capture-MS Homo sapiens
3 TIMD4  
Affinity Capture-MS Homo sapiens
4 UTP11L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 ZNF550  
Affinity Capture-MS Homo sapiens
6 NSL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 TIMM10  
Affinity Capture-MS Homo sapiens
8 EBF2  
Affinity Capture-MS Homo sapiens
9 GOLGA7B  
Affinity Capture-MS Homo sapiens
10 CORO1C 23603
Affinity Capture-MS Homo sapiens
11 FNDC5  
Affinity Capture-MS Homo sapiens
12 HSF2  
Affinity Capture-MS Homo sapiens
13 SRRT 51593
Affinity Capture-MS Homo sapiens
14 FRMD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 CDH1 999
Proximity Label-MS Homo sapiens
16 JOSD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 STK11 6794
Affinity Capture-MS Homo sapiens
18 RCAN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 SNW1 22938
Affinity Capture-MS Homo sapiens
20 GAS2L1 10634
Affinity Capture-MS Homo sapiens
21 FAM213A 84293
Affinity Capture-MS Homo sapiens
22 JAK2 3717
Affinity Capture-MS Homo sapiens
23 CDK11A 728642
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SPRR3 is involved
PathwayEvidenceSource
Developmental Biology TAS Reactome
Formation of the cornified envelope TAS Reactome
Keratinization TAS Reactome





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