Gene description for APOA4
Gene name apolipoprotein A-IV
Gene symbol APOA4
Other names/aliases -
Species Homo sapiens
 Database cross references - APOA4
ExoCarta ExoCarta_337
Vesiclepedia VP_337
Entrez Gene 337
HGNC 602
MIM 107690
 APOA4 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Plasma 36624553    
Plasma 36624553    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Urine 19056867    
 Gene ontology annotations for APOA4
Molecular Function
    lipid transporter activity GO:0005319 TAS
    copper ion binding GO:0005507 IDA
    protein binding GO:0005515 IPI
    phospholipid binding GO:0005543 IBA
    lipid binding GO:0008289 IMP
    antioxidant activity GO:0016209 IDA
    phosphatidylcholine binding GO:0031210 IDA
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
    phosphatidylcholine-sterol O-acyltransferase activator activity GO:0060228 IBA
    phosphatidylcholine-sterol O-acyltransferase activator activity GO:0060228 IDA
    cholesterol transfer activity GO:0120020 IBA
    cholesterol transfer activity GO:0120020 IDA
Biological Process
    innate immune response in mucosa GO:0002227 IDA
    lipid transport GO:0006869 IDA
    response to lipid hydroperoxide GO:0006982 IDA
    leukocyte cell-cell adhesion GO:0007159 IDA
    cholesterol metabolic process GO:0008203 IBA
    cholesterol metabolic process GO:0008203 IDA
    positive regulation of triglyceride catabolic process GO:0010898 IDA
    peripheral nervous system axon regeneration GO:0014012 IEA
    lipid catabolic process GO:0016042 IDA
    removal of superoxide radicals GO:0019430 IDA
    regulation of intestinal cholesterol absorption GO:0030300 IEA
    regulation of cholesterol transport GO:0032374 IDA
    cholesterol efflux GO:0033344 IBA
    cholesterol efflux GO:0033344 IDA
    phospholipid efflux GO:0033700 IBA
    phospholipid efflux GO:0033700 IDA
    response to triglyceride GO:0034014 IEA
    chylomicron remodeling GO:0034371 IC
    very-low-density lipoprotein particle remodeling GO:0034372 IDA
    high-density lipoprotein particle remodeling GO:0034375 IDA
    chylomicron assembly GO:0034378 TAS
    negative regulation of plasma lipoprotein oxidation GO:0034445 IDA
    response to stilbenoid GO:0035634 IEA
    lipoprotein metabolic process GO:0042157 IEA
    cholesterol homeostasis GO:0042632 IDA
    hydrogen peroxide catabolic process GO:0042744 IDA
    reverse cholesterol transport GO:0043691 IDA
    positive regulation of fatty acid biosynthetic process GO:0045723 IDA
    phosphatidylcholine metabolic process GO:0046470 IDA
    positive regulation of lipoprotein lipase activity GO:0051006 IDA
    lipid homeostasis GO:0055088 IDA
    acylglycerol homeostasis GO:0055090 IBA
    protein-lipid complex assembly GO:0065005 IMP
    positive regulation of CoA-transferase activity GO:1905920 IDA
Subcellular Localization
    extracellular region GO:0005576 HDA
    extracellular region GO:0005576 NAS
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IDA
    early endosome GO:0005769 TAS
    endoplasmic reticulum lumen GO:0005788 TAS
    cytosol GO:0005829 TAS
    cell surface GO:0009986 IDA
    very-low-density lipoprotein particle GO:0034361 IBA
    very-low-density lipoprotein particle GO:0034361 IDA
    low-density lipoprotein particle GO:0034362 IBA
    high-density lipoprotein particle GO:0034364 IBA
    high-density lipoprotein particle GO:0034364 IDA
    chylomicron GO:0042627 IBA
    chylomicron GO:0042627 IDA
    synapse GO:0045202 IEA
    collagen-containing extracellular matrix GO:0062023 HDA
    extracellular exosome GO:0070062 HDA
    blood microparticle GO:0072562 HDA
    extracellular vesicle GO:1903561 IBA
 Experiment description of studies that identified APOA4 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 606
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|HSP90|TSG101|GAPDH
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36624553    
Organism Homo sapiens
Experiment description Combination of size-exclusion chromatography and ion exchange adsorption for improving the proteomic analysis of plasma-derived extracellular vesicles
Authors "Wang Y, Zhang Y, Li Z, Wei S, Chi X, Yan X, Lv H, Zhao L, Zhao L. "
Journal name Proteomics
Publication year 2023
Sample Plasma
Sample name Plasma - Ion exchange adsorption 12.5 mg
Isolation/purification methods Filtration
Size exclusion chromatography
Ion exchange adsorption
Centrifugation
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 607
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|HSP90|TSG101|GAPDH
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36624553    
Organism Homo sapiens
Experiment description Combination of size-exclusion chromatography and ion exchange adsorption for improving the proteomic analysis of plasma-derived extracellular vesicles
Authors "Wang Y, Zhang Y, Li Z, Wei S, Chi X, Yan X, Lv H, Zhao L, Zhao L. "
Journal name Proteomics
Publication year 2023
Sample Plasma
Sample name Plasma - Ion exchange adsorption 25 mg
Isolation/purification methods Filtration
Size exclusion chromatography
Ion exchange adsorption
Centrifugation
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for APOA4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CMTM4  
Two-hybrid Homo sapiens
2 MAGEA6  
Two-hybrid Homo sapiens
3 ALB 213
Affinity Capture-MS Homo sapiens
4 GNG8  
Affinity Capture-MS Homo sapiens
5 SNX27 81609
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 MBNL1 4154
Affinity Capture-MS Homo sapiens
7 AGTRAP 57085
Two-hybrid Homo sapiens
8 GPLD1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
9 GDPD1 284161
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 KLK10 5655
Affinity Capture-MS Homo sapiens
11 ST6GALNAC6 30815
Affinity Capture-MS Homo sapiens
12 CMTM5  
Two-hybrid Homo sapiens
13 UBE2U  
Affinity Capture-MS Homo sapiens
14 SOX2  
Affinity Capture-MS Homo sapiens
15 PHF11 51131
Affinity Capture-MS Homo sapiens
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