 Experiment description of studies that identified HIST1H2AK in sEVs |
| 1 |
| Experiment ID |
21 |
| MISEV standards |
|
✔
EM|IEM
|
Biophysical techniques |
|
✔
Alix|TSG101|HSP70|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
19837982
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215. |
| Authors |
"Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson" |
| Journal name |
MCP
|
| Publication year |
2009 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1215 |
| Isolation/purification methods |
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.10-1.12 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [Orbitrap]
Western blotting |
|
|
| 2 |
| Experiment ID |
224 |
| MISEV standards |
|
✔
EM|AFM
|
Biophysical techniques |
|
✔
Alix|TSG101|CD63|CD81
|
Enriched markers |
|
✔
GOLGA2
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25944692
|
| Organism |
Homo sapiens |
| Experiment description |
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes |
| Authors |
"Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S" |
| Journal name |
Oncotarget
|
| Publication year |
2015 |
| Sample |
Neuroblastoma cells |
| Sample name |
SH-SY5Y |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
OptiPrep density gradient |
| Flotation density |
1.10 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 3 |
| Experiment ID |
138 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|HSP90|RAB5|LAMP2|CD9
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
"Hosseini-Beheshti E, Guns ES." |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
DU145 - Rep 2 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 4 |
| Experiment ID |
140 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|HSP90|RAB5|LAMP2|CD9
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
"Hosseini-Beheshti E, Guns ES." |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
VCaP - Rep 2 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 5 |
| Experiment ID |
141 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|HSP90|RAB5|LAMP2|CD9
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
"Hosseini-Beheshti E, Guns ES." |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
VCaP - Rep 3 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 6 |
| Experiment ID |
142 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|HSP90|RAB5|LAMP2|CD9
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
"Hosseini-Beheshti E, Guns ES." |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
LNCaP - Rep 2 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 7 |
| Experiment ID |
144 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|HSP90|RAB5|CD9
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
"Hosseini-Beheshti E, Guns ES." |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
C4-2 - Rep 2 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 8 |
| Experiment ID |
145 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|HSP90|RAB5|CD10
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
"Hosseini-Beheshti E, Guns ES." |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
C4-2 - Rep 3 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 9 |
| Experiment ID |
146 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|HSP90|CD9
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
"Hosseini-Beheshti E, Guns ES." |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
RWPE - Rep 2 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
