Gene description for LIMS2
Gene name LIM and senescent cell antigen-like domains 2
Gene symbol LIMS2
Other names/aliases PINCH-2
PINCH2
Species Homo sapiens
 Database cross references - LIMS2
ExoCarta ExoCarta_55679
Entrez Gene 55679
HGNC 16084
MIM 607908
UniProt Q7Z4I7  
 LIMS2 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for LIMS2
Molecular Function
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
Biological Process
    single organismal cell-cell adhesion GO:0016337 IEA
    negative regulation of hepatocyte proliferation GO:2000346 IEA
    cell junction assembly GO:0034329 TAS
    negative regulation of apoptotic process GO:0043066 IEA
    cell-cell junction organization GO:0045216 IEA
    negative regulation of neural precursor cell proliferation GO:2000178 IEA
    positive regulation of integrin-mediated signaling pathway GO:2001046 IEA
Subcellular Localization
    nucleus GO:0005634 IEA
    plasma membrane GO:0005886 IEA
    focal adhesion GO:0005925 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified LIMS2 in exosomes
1
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for LIMS2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ILK 3611
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which LIMS2 is involved
PathwayEvidenceSource
Cell-extracellular matrix interactions TAS Reactome





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