Gene description for PPAP2C
Gene name phosphatidic acid phosphatase type 2C
Gene symbol PPAP2C
Other names/aliases LPP2
PAP-2c
PAP2-g
Species Homo sapiens
 Database cross references - PPAP2C
ExoCarta ExoCarta_8612
Entrez Gene 8612
HGNC 9230
MIM 607126
UniProt O43688  
 PPAP2C identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
 Gene ontology annotations for PPAP2C
Molecular Function
    phosphatidate phosphatase activity GO:0008195 IEA
    protein binding GO:0005515 IPI
    phosphoprotein phosphatase activity GO:0004721 TAS
Biological Process
    sphingolipid biosynthetic process GO:0030148 TAS
    sphingolipid metabolic process GO:0006665 TAS
    small molecule metabolic process GO:0044281 TAS
    dephosphorylation GO:0016311 TAS
Subcellular Localization
    integral component of membrane GO:0016021 IEA
    membrane GO:0016020 TAS
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified PPAP2C in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PPAP2C
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BAG6 7917
Two-hybrid Homo sapiens
2 UNC119  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which PPAP2C is involved
PathwayEvidenceSource
Sphingolipid de novo biosynthesis TAS Reactome





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