Gene description for PPAP2B
Gene name phosphatidic acid phosphatase type 2B
Gene symbol PPAP2B
Other names/aliases Dri42
LPP3
PAP2B
VCIP
Species Homo sapiens
 Database cross references - PPAP2B
ExoCarta ExoCarta_8613
Entrez Gene 8613
HGNC 9229
MIM 607125
UniProt O14495  
 PPAP2B identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Prostate cancer cells 25844599    
 Gene ontology annotations for PPAP2B
Molecular Function
    phosphoprotein phosphatase activity GO:0004721 TAS
    protein binding GO:0005515 IPI
    phosphatidate phosphatase activity GO:0008195 IEA
    lipid phosphatase activity GO:0042577 IEA
    integrin binding GO:0005178 IEA
    sphingosine-1-phosphate phosphatase activity GO:0042392 IEA
Biological Process
    negative regulation of protein phosphorylation GO:0001933 IDA
    regulation of sphingolipid mediated signaling pathway GO:1902068 IEA
    sphingolipid metabolic process GO:0006665 TAS
    lipid metabolic process GO:0006629 NAS
    phospholipid metabolic process GO:0006644 IEA
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IEA
    canonical Wnt signaling pathway involved in positive regulation of wound healing GO:0044330 IMP
    sphingolipid biosynthetic process GO:0030148 TAS
    canonical Wnt signaling pathway involved in positive regulation of endothelial cell migration GO:0044328 IMP
    homotypic cell-cell adhesion GO:0034109 IDA
    dephosphorylation GO:0016311 TAS
    regulation of Wnt signaling pathway GO:0030111 IEA
    positive regulation of sequence-specific DNA binding transcription factor activity GO:0051091 IDA
    blood vessel development GO:0001568 IEA
    gastrulation with mouth forming second GO:0001702 IEA
    Bergmann glial cell differentiation GO:0060020 IEA
    germ cell migration GO:0008354 TAS
    protein stabilization GO:0050821 IDA
    canonical Wnt signaling pathway involved in positive regulation of cell-cell adhesion GO:0044329 IMP
    small molecule metabolic process GO:0044281 TAS
Subcellular Localization
    adherens junction GO:0005912 TAS
    plasma membrane GO:0005886 TAS
    integral component of membrane GO:0016021 IEA
    extracellular exosome GO:0070062 IDA
    membrane GO:0016020 TAS
    Golgi apparatus GO:0005794 IEA
 Experiment description of studies that identified PPAP2B in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 275
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for PPAP2B
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which PPAP2B is involved
PathwayEvidenceSource
Sphingolipid de novo biosynthesis TAS Reactome





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