Gene description for AQP5
Gene name aquaporin 5
Gene symbol AQP5
Other names/aliases AQP-5
PPKB
Species Homo sapiens
 Database cross references - AQP5
ExoCarta ExoCarta_362
Entrez Gene 362
HGNC 638
MIM 600442
UniProt P55064  
 AQP5 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Saliva 19199708    
 Gene ontology annotations for AQP5
Molecular Function
    protein binding GO:0005515 IPI
    water channel activity GO:0015250 IDA
    glycerol channel activity GO:0015254 IBA
Biological Process
    transmembrane transport GO:0055085 TAS
    odontogenesis GO:0042476 IEP
    glycerol transport GO:0015793 IBA
    ion transmembrane transport GO:0034220 IBA
    water transport GO:0006833 TAS
    pancreatic juice secretion GO:0030157 IEP
    cellular water homeostasis GO:0009992 IBA
    saliva secretion GO:0046541 IEA
    excretion GO:0007588 TAS
    camera-type eye morphogenesis GO:0048593 IEA
    carbon dioxide transport GO:0015670 IDA
Subcellular Localization
    basal plasma membrane GO:0009925 IEA
    plasma membrane GO:0005886 TAS
    apical plasma membrane GO:0016324 IDA
    extracellular exosome GO:0070062 IDA
    integral component of plasma membrane GO:0005887 TAS
    microvillus GO:0005902 IEA
    endoplasmic reticulum GO:0005783 IEA
 Experiment description of studies that identified AQP5 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 66
ISEV standards
IEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD63|CD81
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for AQP5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MDFI  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which AQP5 is involved
PathwayEvidenceSource
Passive transport by Aquaporins TAS Reactome





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