Experiment description of studies that identified TMEM8A in sEVs

1 Experiment ID 1203 MISEV standards ✔ EM Biophysical techniques ✔ SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 34887515     Organism Homo sapiens Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." Journal name Nat Cell Biol Publication year 2021 Sample Colorectal cancer cells Sample name DiFi Isolation/purification methods Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting Mass spectrometry RNA sequencing

2 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for TMEM8A

Protein Interactor ExoCarta ID Identification method PubMed Species 1 KRTAP10-7   Two-hybrid Homo sapiens 2 SPPL2B 56928 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 3 GALNT10 55568 Affinity Capture-MS Homo sapiens 4 KRTAP12-3   Two-hybrid Homo sapiens 5 TMEM106A   Affinity Capture-MS Homo sapiens 6 CMTM5   Affinity Capture-MS Homo sapiens 7 LDLRAD1   Affinity Capture-MS Homo sapiens 8 NOTCH2NL 388677 Two-hybrid Homo sapiens 9 REL 5966 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 10 ASIC4   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 11 NBPF19   Two-hybrid Homo sapiens 12 ATP1B4   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 13 TMPRSS11B 132724 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 14 KRTAP1-1   Two-hybrid Homo sapiens 15 KRTAP5-9   Two-hybrid Homo sapiens 16 KRTAP10-3   Two-hybrid Homo sapiens 17 DDX58 23586 Affinity Capture-RNA Homo sapiens 18 HNRNPK 3190 Two-hybrid Homo sapiens 19 CLEC12B   Affinity Capture-MS Homo sapiens 20 CYSRT1 375791 Two-hybrid Homo sapiens 21 KRTAP10-8   Two-hybrid Homo sapiens 22 TCTN2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 23 KLRC1   Affinity Capture-MS Homo sapiens 24 PCDHGC5   Affinity Capture-MS Homo sapiens 25 KRTAP6-3   Two-hybrid Homo sapiens View the network

Pathways in which TMEM8A is involved

No pathways found