Gene description for PCSK5
Gene name proprotein convertase subtilisin/kexin type 5
Gene symbol PCSK5
Other names/aliases PC5
PC6
PC6A
SPC6
Species Homo sapiens
 Database cross references - PCSK5
ExoCarta ExoCarta_5125
Vesiclepedia VP_5125
Entrez Gene 5125
HGNC 8747
MIM 600488
UniProt Q92824  
 PCSK5 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for PCSK5
Molecular Function
    endopeptidase activity GO:0004175 EXP
    serine-type endopeptidase activity GO:0004252 IBA
    serine-type endopeptidase activity GO:0004252 IDA
    protein binding GO:0005515 IPI
    peptidase activity GO:0008233 IDA
    peptide binding GO:0042277 ISS
Biological Process
    kidney development GO:0001822 IMP
    kidney development GO:0001822 ISS
    renin secretion into blood stream GO:0002001 IEP
    signal peptide processing GO:0006465 IDA
    cell-cell signaling GO:0007267 TAS
    heart development GO:0007507 ISS
    embryo implantation GO:0007566 ISS
    anterior/posterior pattern specification GO:0009952 IMP
    protein processing GO:0016485 IDA
    protein processing GO:0016485 IDA
    peptide hormone processing GO:0016486 IBA
    peptide hormone processing GO:0016486 IDA
    viral life cycle GO:0019058 IEP
    viral life cycle GO:0019058 ISS
    respiratory tube development GO:0030323 ISS
    plasma lipoprotein particle remodeling GO:0034369 TAS
    limb morphogenesis GO:0035108 ISS
    peptide biosynthetic process GO:0043043 IDA
    embryonic digestive tract development GO:0048566 IMP
    embryonic digestive tract development GO:0048566 ISS
    embryonic skeletal system development GO:0048706 IMP
    embryonic skeletal system development GO:0048706 ISS
    cytokine precursor processing GO:0140447 ISS
Subcellular Localization
    Golgi membrane GO:0000139 IBA
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 ISS
    Golgi apparatus GO:0005794 IDA
    Golgi apparatus GO:0005794 TAS
    Golgi lumen GO:0005796 TAS
    trans-Golgi network GO:0005802 IBA
 Experiment description of studies that identified PCSK5 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PCSK5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LY86  
Affinity Capture-MS Homo sapiens
2 KRTAP10-7  
Two-hybrid Homo sapiens
3 CCL3  
Affinity Capture-MS Homo sapiens
4 LY6H  
Affinity Capture-MS Homo sapiens
5 NOTCH2NL 388677
Two-hybrid Homo sapiens
6 GPIHBP1  
Affinity Capture-MS Homo sapiens
7 NAAA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 CACNA1A  
Two-hybrid Homo sapiens
9 PRG3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 PI15  
Affinity Capture-MS Homo sapiens
11 KRTAP10-8  
Two-hybrid Homo sapiens
12 NUFIP2 57532
Two-hybrid Homo sapiens
13 PLAUR 5329
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 PRG2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 SPSB4  
Affinity Capture-MS Homo sapiens
16 CLEC12B  
Affinity Capture-MS Homo sapiens
17 CDH23 64072
Affinity Capture-MS Homo sapiens
18 KRTAP5-9  
Two-hybrid Homo sapiens
19 KRTAP10-3  
Two-hybrid Homo sapiens
20 SLURP1  
Affinity Capture-MS Homo sapiens
21 ATN1  
Two-hybrid Homo sapiens
22 SLAMF1 6504
Affinity Capture-MS Homo sapiens
23 INSL5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 Hoxa1  
Two-hybrid Mus musculus
PCA Mus musculus
25 APPBP2  
Two-hybrid Homo sapiens
26 LRP1 4035
Affinity Capture-MS Homo sapiens
27 CELA3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 KRTAP4-12  
Two-hybrid Homo sapiens
29 SDF2L1 23753
Affinity Capture-MS Homo sapiens
30 LCE3C  
Two-hybrid Homo sapiens
31 STK16 8576
Two-hybrid Homo sapiens
32 FBXO2 26232
Affinity Capture-MS Homo sapiens
33 CD160  
Affinity Capture-MS Homo sapiens
34 C1orf54  
Affinity Capture-MS Homo sapiens
35 NCR3  
Affinity Capture-MS Homo sapiens
36 GLRX3 10539
Two-hybrid Homo sapiens
37 DNAJB9 4189
Affinity Capture-MS Homo sapiens
38 CEACAM8 1088
Affinity Capture-MS Homo sapiens
39 MEOX2  
Two-hybrid Homo sapiens
40 PTPRK 5796
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 LYPD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 CST9L  
Affinity Capture-MS Homo sapiens
43 GPHA2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 ST14 6768
Affinity Capture-MS Homo sapiens
45 IL5RA  
Affinity Capture-MS Homo sapiens
46 CFC1  
Affinity Capture-MS Homo sapiens
47 LYPD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 IGFL3  
Affinity Capture-MS Homo sapiens
49 PLA2G10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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