Gene description for ACY3
Gene name aminoacylase 3
Gene symbol ACY3
Other names/aliases ACY-3
HCBP1
Species Homo sapiens
 Database cross references - ACY3
ExoCarta ExoCarta_91703
Vesiclepedia VP_91703
Entrez Gene 91703
HGNC 24104
MIM 614413
UniProt Q96HD9  
 ACY3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Urine 19056867    
 Gene ontology annotations for ACY3
Molecular Function
    aminoacylase activity GO:0004046 IBA
    aminoacylase activity GO:0004046 ISS
    protein binding GO:0005515 IPI
    hydrolase activity, acting on ester bonds GO:0016788 IEA
    identical protein binding GO:0042802 IEA
    metal ion binding GO:0046872 IEA
Subcellular Localization
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
    apical plasma membrane GO:0016324 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified ACY3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for ACY3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FAM166A  
Affinity Capture-MS Homo sapiens
2 NOTCH2NL 388677
Two-hybrid Homo sapiens
3 PIAS2  
Two-hybrid Homo sapiens
4 KRTAP10-8  
Two-hybrid Homo sapiens
5 MCOLN3  
Affinity Capture-MS Homo sapiens
6 INCA1  
Two-hybrid Homo sapiens
7 PLEKHF2 79666
Two-hybrid Homo sapiens
8 KRTAP15-1  
Two-hybrid Homo sapiens
9 MAP3K8  
Two-hybrid Homo sapiens
10 KRTAP10-3  
Two-hybrid Homo sapiens
11 DDIT4L  
Two-hybrid Homo sapiens
12 PMM2 5373
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
13 RRAS 6237
Two-hybrid Homo sapiens
14 KRTAP26-1  
Two-hybrid Homo sapiens
15 RWDD2B  
Affinity Capture-MS Homo sapiens
16 ASPA  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
17 CYSRT1 375791
Two-hybrid Homo sapiens
18 NPDC1  
Affinity Capture-MS Homo sapiens
19 GORASP2 26003
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
20 ACY3 91703
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
21 AAMDC  
Two-hybrid Homo sapiens
22 MORF4L1  
Affinity Capture-MS Homo sapiens
23 PTGER4  
Two-hybrid Homo sapiens
24 LNX1  
Two-hybrid Homo sapiens
25 NEDD4L 23327
Two-hybrid Homo sapiens
26 RARG  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which ACY3 is involved
PathwayEvidenceSource
Aflatoxin activation and detoxification TAS Reactome
Biological oxidations TAS Reactome
Metabolism TAS Reactome





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