Gene description for PMM2
Gene name phosphomannomutase 2
Gene symbol PMM2
Other names/aliases CDG1
CDG1a
CDGS
PMI
PMI1
PMM 2
Species Homo sapiens
 Database cross references - PMM2
ExoCarta ExoCarta_5373
Entrez Gene 5373
HGNC 9115
MIM 601785
UniProt O15305  
 PMM2 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for PMM2
Molecular Function
    phosphomannomutase activity GO:0004615 IEA
    protein binding GO:0005515 IPI
Biological Process
    GDP-mannose biosynthetic process GO:0009298 TAS
    protein N-linked glycosylation via asparagine GO:0018279 TAS
    dolichol-linked oligosaccharide biosynthetic process GO:0006488 TAS
    cellular protein metabolic process GO:0044267 TAS
    post-translational protein modification GO:0043687 TAS
    protein glycosylation GO:0006486 TAS
    mannose biosynthetic process GO:0019307 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 IDA
    neuronal cell body GO:0043025 IEA
 Experiment description of studies that identified PMM2 in exosomes
1
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PMM2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which PMM2 is involved
PathwayEvidenceSource
Synthesis of GDP-mannose TAS Reactome





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