Gene description for GMPPA
Gene name GDP-mannose pyrophosphorylase A
Gene symbol GMPPA
Other names/aliases AAMR
Species Homo sapiens
 Database cross references - GMPPA
ExoCarta ExoCarta_29926
Entrez Gene 29926
HGNC 22923
MIM 615495
UniProt Q96IJ6  
 GMPPA identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for GMPPA
Molecular Function
    nucleotidyltransferase activity GO:0016779 IEA
    protein binding GO:0005515 IPI
Biological Process
    protein N-linked glycosylation via asparagine GO:0018279 TAS
    cellular protein metabolic process GO:0044267 TAS
    post-translational protein modification GO:0043687 TAS
    dolichol-linked oligosaccharide biosynthetic process GO:0006488 TAS
    GDP-mannose biosynthetic process GO:0009298 TAS
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    cytoplasm GO:0005737 IEA
 Experiment description of studies that identified GMPPA in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GMPPA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GMPPB 29925
Invivo Homo sapiens
Two-hybrid Homo sapiens
Invivo Homo sapiens
View the network image/svg+xml
 Pathways in which GMPPA is involved
PathwayEvidenceSource
Synthesis of GDP-mannose TAS Reactome





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