Gene description for MPHOSPH8
Gene name M-phase phosphoprotein 8
Gene symbol MPHOSPH8
Other names/aliases HSMPP8
TWA3
mpp8
Species Homo sapiens
 Database cross references - MPHOSPH8
ExoCarta ExoCarta_54737
Vesiclepedia VP_54737
Entrez Gene 54737
HGNC 29810
MIM 611626
UniProt Q99549  
 MPHOSPH8 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for MPHOSPH8
Molecular Function
    protein binding GO:0005515 IPI
    methylated histone binding GO:0035064 IDA
Biological Process
    transcription, DNA-templated GO:0006351 IEA
    regulation of DNA methylation GO:0044030 IMP
    negative regulation of transcription, DNA-templated GO:0045892 IMP
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    nucleolus GO:0005730 IDA
    nucleoplasm GO:0005654 IDA
    nuclear heterochromatin GO:0005720 IDA
    nucleus GO:0005634 IDA
    plasma membrane GO:0005886 IDA
    nuclear nucleosome GO:0000788 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified MPHOSPH8 in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for MPHOSPH8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RANBP9 10048
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which MPHOSPH8 is involved
No pathways found





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