Gene description for PARP10
Gene name poly (ADP-ribose) polymerase family, member 10
Gene symbol PARP10
Other names/aliases ARTD10
Species Homo sapiens
 Database cross references - PARP10
ExoCarta ExoCarta_84875
Vesiclepedia VP_84875
Entrez Gene 84875
HGNC 25895
MIM 609564
UniProt Q53GL7  
 PARP10 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
Thymus 23844026    
 Gene ontology annotations for PARP10
Molecular Function
    transcription corepressor activity GO:0003714 IBA
    NAD+-protein poly-ADP-ribosyltransferase activity GO:0003950 IBA
    NAD+-protein poly-ADP-ribosyltransferase activity GO:0003950 IMP
    NAD+-protein poly-ADP-ribosyltransferase activity GO:0003950 TAS
    protein binding GO:0005515 IPI
    nucleotidyltransferase activity GO:0016779 IEA
    K63-linked polyubiquitin modification-dependent protein binding GO:0070530 IMP
    DNA-binding transcription factor binding GO:0140297 IPI
    NAD+-protein-lysine ADP-ribosyltransferase activity GO:0140804 IEA
    NAD+-protein-aspartate ADP-ribosyltransferase activity GO:0140806 IEA
    NAD+-histone H2BE35 glutamate ADP-ribosyltransferase activity GO:0140822 IEA
    NAD+-histone H2BE2 glutamate ADP-ribosyltransferase activity GO:0140844 IEA
    NAD+-histone H2BE18 glutamate ADP-ribosyltransferase activity GO:0140867 IEA
    NAD+-protein ADP-ribosyltransferase activity GO:1990404 IBA
    NAD+-protein ADP-ribosyltransferase activity GO:1990404 IDA
    NAD+-protein ADP-ribosyltransferase activity GO:1990404 IMP
Biological Process
    chromatin organization GO:0006325 IDA
    negative regulation of gene expression GO:0010629 IBA
    negative regulation of gene expression GO:0010629 IMP
    viral protein processing GO:0019082 TAS
    translesion synthesis GO:0019985 IDA
    negative regulation of NF-kappaB transcription factor activity GO:0032088 IMP
    NAD biosynthesis via nicotinamide riboside salvage pathway GO:0034356 TAS
    negative regulation of DNA-templated transcription GO:0045892 IEA
    negative regulation of fibroblast proliferation GO:0048147 IDA
    protein poly-ADP-ribosylation GO:0070212 IDA
    protein poly-ADP-ribosylation GO:0070212 IMP
    protein auto-ADP-ribosylation GO:0070213 IDA
    protein auto-ADP-ribosylation GO:0070213 IMP
    negative regulation of protein K63-linked ubiquitination GO:1900045 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified PARP10 in exosomes
1
Experiment ID 126
MISEV standards
Biophysical techniques
GAPDH
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
2
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PARP10
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sars 20226
Affinity Capture-MS Mus musculus
2 HIST2H2AC 8338
Biochemical Activity Homo sapiens
3 PCNA 5111
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
4 CDK2 1017
Affinity Capture-MS Homo sapiens
5 UBC 7316
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
6 RASSF8 11228
Affinity Capture-MS Homo sapiens
7 Dclre1b  
Affinity Capture-MS Mus musculus
8 SQSTM1 8878
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
9 MYC  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
10 HIST1H3A 8350
Biochemical Activity Homo sapiens
11 Lyplal1  
Affinity Capture-MS Mus musculus
12 FBXO7 25793
Affinity Capture-MS Homo sapiens
13 GAK 2580
Affinity Capture-MS Homo sapiens
14 HIST2H2BE 8349
Biochemical Activity Homo sapiens
15 B4galt1  
Affinity Capture-MS Mus musculus
16 HIST1H4A 8359
Biochemical Activity Homo sapiens
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