Gene description for EGLN1
Gene name egl-9 family hypoxia-inducible factor 1
Gene symbol EGLN1
Other names/aliases C1orf12
ECYT3
HALAH
HIF-PH2
HIFPH2
HPH-2
HPH2
PHD2
SM20
ZMYND6
Species Homo sapiens
 Database cross references - EGLN1
ExoCarta ExoCarta_54583
Vesiclepedia VP_54583
Entrez Gene 54583
HGNC 1232
MIM 606425
UniProt Q9GZT9  
 EGLN1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for EGLN1
Molecular Function
    enzyme binding GO:0019899 ISS
    peptidyl-proline dioxygenase activity GO:0031543 TAS
    peptidyl-proline 4-dioxygenase activity GO:0031545 IDA
    protein binding GO:0005515 IPI
    oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors GO:0016706 IDA
    iron ion binding GO:0005506 IEA
    L-ascorbic acid binding GO:0031418 IEA
Biological Process
    negative regulation of cyclic-nucleotide phosphodiesterase activity GO:0051344 ISS
    negative regulation of cAMP catabolic process GO:0030821 ISS
    labyrinthine layer development GO:0060711 IEA
    oxygen homeostasis GO:0032364 IDA
    cardiac muscle tissue morphogenesis GO:0055008 IEA
    response to hypoxia GO:0001666 IDA
    ventricular septum morphogenesis GO:0060412 IEA
    regulation of transcription from RNA polymerase II promoter in response to hypoxia GO:0061418 TAS
    cellular response to hypoxia GO:0071456 TAS
    regulation of angiogenesis GO:0045765 ISS
    peptidyl-proline hydroxylation to 4-hydroxy-L-proline GO:0018401 IDA
    heart trabecula formation GO:0060347 IEA
    oxidation-reduction process GO:0055114 IEA
    negative regulation of sequence-specific DNA binding transcription factor activity GO:0043433 IDA
    response to nitric oxide GO:0071731 IDA
Subcellular Localization
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 IEA
 Experiment description of studies that identified EGLN1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for EGLN1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which EGLN1 is involved
PathwayEvidenceSource
Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha TAS Reactome





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