Gene description for GPAM
Gene name glycerol-3-phosphate acyltransferase, mitochondrial
Gene symbol GPAM
Other names/aliases GPAT
GPAT1
Species Homo sapiens
 Database cross references - GPAM
ExoCarta ExoCarta_57678
Vesiclepedia VP_57678
Entrez Gene 57678
HGNC 24865
MIM 602395
UniProt Q9HCL2  
 GPAM identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for GPAM
Molecular Function
    glycerol-3-phosphate O-acyltransferase activity GO:0004366 EXP
    glycerol-3-phosphate O-acyltransferase activity GO:0004366 IBA
    glycerol-3-phosphate O-acyltransferase activity GO:0004366 IMP
    glycerol-3-phosphate O-acyltransferase activity GO:0004366 ISS
    protein binding GO:0005515 IPI
Biological Process
    regulation of cytokine production GO:0001817 IEA
    fatty acid metabolic process GO:0006631 IEA
    acyl-CoA metabolic process GO:0006637 IEA
    glycerophospholipid metabolic process GO:0006650 IBA
    diacylglycerol biosynthetic process GO:0006651 ISS
    phosphatidic acid biosynthetic process GO:0006654 ISS
    phosphatidic acid biosynthetic process GO:0006654 TAS
    phosphatidylglycerol biosynthetic process GO:0006655 IMP
    activation-induced cell death of T cells GO:0006924 IEA
    response to glucose GO:0009749 IEA
    CDP-diacylglycerol biosynthetic process GO:0016024 IEA
    triglyceride biosynthetic process GO:0019432 IBA
    triglyceride biosynthetic process GO:0019432 IDA
    triglyceride biosynthetic process GO:0019432 TAS
    positive regulation of multicellular organism growth GO:0040018 IEA
    positive regulation of activated T cell proliferation GO:0042104 IEA
    activated T cell proliferation GO:0050798 IEA
    defense response to virus GO:0051607 IEA
    fatty acid homeostasis GO:0055089 IEA
    phospholipid homeostasis GO:0055091 IEA
    negative regulation of activation-induced cell death of T cells GO:0070236 IEA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrial outer membrane GO:0005741 TAS
    plasma membrane GO:0005886 IEA
 Experiment description of studies that identified GPAM in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GPAM
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ECH1 1891
Affinity Capture-MS Homo sapiens
2 Mcm3 17215
Affinity Capture-MS Mus musculus
3 SCIN 85477
Affinity Capture-MS Homo sapiens
4 MICAL1 64780
Affinity Capture-MS Homo sapiens
5 CADM4 199731
Affinity Capture-MS Homo sapiens
6 CDH5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 MTCH2 23788
Proximity Label-MS Homo sapiens
8 Rcc1  
Affinity Capture-MS Mus musculus
9 GPAM 57678
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 HDAC1 3065
Affinity Capture-MS Homo sapiens
11 CLU 1191
Affinity Capture-MS Homo sapiens
12 APP 351
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
13 OCIAD1 54940
Proximity Label-MS Homo sapiens
14 IDE 3416
Affinity Capture-MS Homo sapiens
15 FIS1 51024
Proximity Label-MS Homo sapiens
16 ATP2B2 491
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 TBC1D24 57465
Affinity Capture-MS Homo sapiens
18 SIRT2 22933
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 SRC 6714
Affinity Capture-MS Homo sapiens
20 CIDEB  
Two-hybrid Homo sapiens
21 PSMC4 5704
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 Bub1  
Affinity Capture-MS Mus musculus
23 AKAP1 8165
Proximity Label-MS Homo sapiens
View the network image/svg+xml



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