Gene description for GLUD2
Gene name glutamate dehydrogenase 2
Gene symbol GLUD2
Other names/aliases GDH2
GLUDP1
Species Homo sapiens
 Database cross references - GLUD2
ExoCarta ExoCarta_2747
Vesiclepedia VP_2747
Entrez Gene 2747
HGNC 4336
MIM 300144
UniProt P49448  
 GLUD2 identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
 Gene ontology annotations for GLUD2
Molecular Function
    glutamate dehydrogenase (NAD+) activity GO:0004352 IBA
    glutamate dehydrogenase (NAD+) activity GO:0004352 IDA
    glutamate dehydrogenase [NAD(P)+] activity GO:0004353 IDA
    glutamate dehydrogenase (NADP+) activity GO:0004354 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IDA
    ADP binding GO:0043531 IDA
    L-leucine binding GO:0070728 IDA
Biological Process
    glutamate metabolic process GO:0006536 IDA
    glutamate biosynthetic process GO:0006537 IDA
    glutamate catabolic process GO:0006538 IBA
    glutamate catabolic process GO:0006538 IDA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 IEA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified GLUD2 in sEVs
1
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GLUD2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPN1 6184
Proximity Label-MS Homo sapiens
2 CRBN  
Affinity Capture-MS Homo sapiens
3 GLUD1 2746
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 DHFRL1  
Proximity Label-MS Homo sapiens
5 ATF2  
Affinity Capture-MS Homo sapiens
6 KLHL22 84861
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 RNF2  
Affinity Capture-MS Homo sapiens
8 C9orf72  
Affinity Capture-MS Homo sapiens
9 RFPL4B  
Affinity Capture-MS Homo sapiens
10 HYPK 25764
Affinity Capture-MS Homo sapiens
11 TSPAN5 10098
Affinity Capture-MS Homo sapiens
12 TOM1L1 10040
Two-hybrid Homo sapiens
13 MOV10 4343
Affinity Capture-RNA Homo sapiens
14 NPM1 4869
Proximity Label-MS Homo sapiens
15 MCM2 4171
Affinity Capture-MS Homo sapiens
16 SHMT2 6472
Affinity Capture-RNA Homo sapiens
17 C21orf33  
Affinity Capture-MS Homo sapiens
18 PTGR2 145482
Co-fractionation Homo sapiens
View the network image/svg+xml



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